Improved dsRNA isolation and purification method validated by viral dsRNA detection using novel primers in Saccharomyces cerevisiae

Improved dsRNA isolation and purification method validated by viral dsRNA detection using novel primers in Saccharomyces cerevisiae

Accurate genomic sequencing demands high-quality double-stranded RNA (dsRNA). Existing methods for dsRNA extraction from yeast, fungi, and plants primarily rely on cellulose, suitable only for small volume extractions, or the time-consuming lithium chloride precipitation. To streamline the tradition...

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Bibliographic Details
Main Authors: Fernando M.H. Cardoso, Alexandre Elias, Inês Pereira, Isabel Maurício, Olga Matos
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:MethodsX
Subjects:
Viral dsRNA
Phenol extraction
Ammonium sulphate
RT-PCR
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016123004314

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http://www.sciencedirect.com/science/article/pii/S2215016123004314

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