High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications
Abstract Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-04859-2 |
| _version_ | 1827952611789111296 |
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| author | Claudio L. Alter Pascal Detampel Roman B. Schefer Claudia Lotter Patrick Hauswirth Ramya D. Puligilla Vera J. Weibel Susanne H. Schenk Wolf Heusermann Melanie Schürz Nicole Meisner-Kober Cornelia Palivan Tomaž Einfalt Jörg Huwyler |
| author_facet | Claudio L. Alter Pascal Detampel Roman B. Schefer Claudia Lotter Patrick Hauswirth Ramya D. Puligilla Vera J. Weibel Susanne H. Schenk Wolf Heusermann Melanie Schürz Nicole Meisner-Kober Cornelia Palivan Tomaž Einfalt Jörg Huwyler |
| author_sort | Claudio L. Alter |
| collection | DOAJ |
| description | Abstract Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV-based pharmaceutical therapeutics. |
| first_indexed | 2024-04-09T14:00:21Z |
| format | Article |
| id | doaj.art-be10fbb715fc4794919ad22eb4023b74 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-09T14:00:21Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-be10fbb715fc4794919ad22eb4023b742023-05-07T11:20:55ZengNature PortfolioCommunications Biology2399-36422023-05-016111710.1038/s42003-023-04859-2High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applicationsClaudio L. Alter0Pascal Detampel1Roman B. Schefer2Claudia Lotter3Patrick Hauswirth4Ramya D. Puligilla5Vera J. Weibel6Susanne H. Schenk7Wolf Heusermann8Melanie Schürz9Nicole Meisner-Kober10Cornelia Palivan11Tomaž Einfalt12Jörg Huwyler13Department of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselImaging Core Facility, University of BaselDepartment of Biosciences & Medical Biology, University of SalzburgDepartment of Biosciences & Medical Biology, University of SalzburgDepartment of Chemistry, University of BaselDepartment of Pharmaceutical Technology, University of BaselDepartment of Pharmaceutical Technology, University of BaselAbstract Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell membrane blebbing and apoptotic body secretion induced by chemical stressors. nPMVs showed no significant differences compared to native EVs from the same cell line in cryo-TEM analysis, in vitro cellular interactions, and in vivo biodistribution studies in zebrafish larvae. Proteomics and lipidomics, on the other hand, suggested substantial differences consistent with the divergent origin of these two EV types and indicated that nPMVs primarily derive from apoptotic extracellular vesicles. nPMVs may provide an attractive source for developing EV-based pharmaceutical therapeutics.https://doi.org/10.1038/s42003-023-04859-2 |
| spellingShingle | Claudio L. Alter Pascal Detampel Roman B. Schefer Claudia Lotter Patrick Hauswirth Ramya D. Puligilla Vera J. Weibel Susanne H. Schenk Wolf Heusermann Melanie Schürz Nicole Meisner-Kober Cornelia Palivan Tomaž Einfalt Jörg Huwyler High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications Communications Biology |
| title | High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| title_full | High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| title_fullStr | High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| title_full_unstemmed | High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| title_short | High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| title_sort | high efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications |
| url | https://doi.org/10.1038/s42003-023-04859-2 |
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