The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms
Recombinant human peroxiredoxin-5 (hPRDX5), isolated from anti-cancer bioactive peptide (ACBPs), shows a homology of 89% with goat peroxiredoxin-5 (gPRDX5) and is reported to display anti-tumor activity in vivo. Herein, we explored the effect of hPRDX5 and the responsible mechanism in treating pancr...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Associação Brasileira de Divulgação Científica
2022-09-01
|
| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100658&tlng=en |
| _version_ | 1828176330572693504 |
|---|---|
| author | Lihua Cui Yuanyuan Jin Sen Zou Jing Xun Xiangyang Yu Qi Zhang Zhaoyong Yang |
| author_facet | Lihua Cui Yuanyuan Jin Sen Zou Jing Xun Xiangyang Yu Qi Zhang Zhaoyong Yang |
| author_sort | Lihua Cui |
| collection | DOAJ |
| description | Recombinant human peroxiredoxin-5 (hPRDX5), isolated from anti-cancer bioactive peptide (ACBPs), shows a homology of 89% with goat peroxiredoxin-5 (gPRDX5) and is reported to display anti-tumor activity in vivo. Herein, we explored the effect of hPRDX5 and the responsible mechanism in treating pancreatic cancer. Tumor-bearing mice were randomly divided into normal PBS group and treatment group (n=5; 10 mg/kg hPRDX5). Flow cytometry was employed to examine lymphocytes, myeloid-derived suppressor cell subsets, and the function proteins of natural killer (NK) cells in peripheral blood, spleen, and tumor tissues of mice. Western blot was used to measure the protein expressions of the key nodes in TLR4-MAPK-NF-κB signaling pathway. The rate of tumor suppression was 57.6% at a 10 mg/kg dose in orthotopic transplanted tumor mice. Moreover, the population of CD3+CD4+T cells, NK cells, and CD3+CD8+T cells was significantly increased in the tumor tissue of the hPRDX5 group, while the proportion of granulocytic-myeloid-derived suppressor cells decreased slightly. In addition, after treatment with hPRDX5, the percentage of NK cells in blood increased more than 4-fold. Our findings indicated that hPRDX5 effectively suppressed pancreatic cancer possibly via the TLR4-MAPK-NF-κB signaling cascade; hence hPRDX5 could be a prospective immunotherapy candidate for treating pancreatic cancer. |
| first_indexed | 2024-04-12T04:42:29Z |
| format | Article |
| id | doaj.art-be13ba6840f34c78b448c0b383515b2a |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-12T04:42:29Z |
| publishDate | 2022-09-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-be13ba6840f34c78b448c0b383515b2a2022-12-22T03:47:35ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2022-09-015510.1590/1414-431x2022e12324The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanismsLihua Cuihttps://orcid.org/0000-0002-7921-4225Yuanyuan Jinhttps://orcid.org/0000-0002-1284-9736Sen Zouhttps://orcid.org/0000-0002-6593-0618Jing Xunhttps://orcid.org/0000-0002-8730-4212Xiangyang Yuhttps://orcid.org/0000-0002-3688-347XQi Zhanghttps://orcid.org/0000-0002-7817-2211Zhaoyong Yanghttps://orcid.org/0000-0001-7197-3991Recombinant human peroxiredoxin-5 (hPRDX5), isolated from anti-cancer bioactive peptide (ACBPs), shows a homology of 89% with goat peroxiredoxin-5 (gPRDX5) and is reported to display anti-tumor activity in vivo. Herein, we explored the effect of hPRDX5 and the responsible mechanism in treating pancreatic cancer. Tumor-bearing mice were randomly divided into normal PBS group and treatment group (n=5; 10 mg/kg hPRDX5). Flow cytometry was employed to examine lymphocytes, myeloid-derived suppressor cell subsets, and the function proteins of natural killer (NK) cells in peripheral blood, spleen, and tumor tissues of mice. Western blot was used to measure the protein expressions of the key nodes in TLR4-MAPK-NF-κB signaling pathway. The rate of tumor suppression was 57.6% at a 10 mg/kg dose in orthotopic transplanted tumor mice. Moreover, the population of CD3+CD4+T cells, NK cells, and CD3+CD8+T cells was significantly increased in the tumor tissue of the hPRDX5 group, while the proportion of granulocytic-myeloid-derived suppressor cells decreased slightly. In addition, after treatment with hPRDX5, the percentage of NK cells in blood increased more than 4-fold. Our findings indicated that hPRDX5 effectively suppressed pancreatic cancer possibly via the TLR4-MAPK-NF-κB signaling cascade; hence hPRDX5 could be a prospective immunotherapy candidate for treating pancreatic cancer.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100658&tlng=enRecombinant human peroxiredoxin-5Pancreatic cancerImmunotherapyImmune cells activationMyeloid-derived suppressor cells |
| spellingShingle | Lihua Cui Yuanyuan Jin Sen Zou Jing Xun Xiangyang Yu Qi Zhang Zhaoyong Yang The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms Brazilian Journal of Medical and Biological Research Recombinant human peroxiredoxin-5 Pancreatic cancer Immunotherapy Immune cells activation Myeloid-derived suppressor cells |
| title | The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms |
| title_full | The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms |
| title_fullStr | The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms |
| title_full_unstemmed | The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms |
| title_short | The antitumor activity of hPRDX5 against pancreatic cancer and the possible mechanisms |
| title_sort | antitumor activity of hprdx5 against pancreatic cancer and the possible mechanisms |
| topic | Recombinant human peroxiredoxin-5 Pancreatic cancer Immunotherapy Immune cells activation Myeloid-derived suppressor cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2022000100658&tlng=en |
| work_keys_str_mv | AT lihuacui theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT yuanyuanjin theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT senzou theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT jingxun theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT xiangyangyu theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT qizhang theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT zhaoyongyang theantitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT lihuacui antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT yuanyuanjin antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT senzou antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT jingxun antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT xiangyangyu antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT qizhang antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms AT zhaoyongyang antitumoractivityofhprdx5againstpancreaticcancerandthepossiblemechanisms |