Visual quantification of prostaglandin E2 discharge from a single cell

Visual quantification of prostaglandin E2 discharge from a single cell

Calcium transients drive cells to discharge prostaglandin E2 (PGE2). We visualized PGE2-induced protein kinase A (PKA) activation and quantitated PGE2 secreted from a single cell by combining fluorescence microscopy and a simulation model. For this purpose, we first prepared PGE2-producer cells that...

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Bibliographic Details
Main Authors: Tetsuya Watabe, Shinya Yamahira, Michiyuki Matsuda, Kenta Terai
Format: Article
Language:English
Published: Japan Society for Cell Biology 2023-10-01
Series:Cell Structure and Function
Subjects:
prostaglandin e2
imaging
intercellular communication
biosensor
quantification
Online Access:https://www.jstage.jst.go.jp/article/csf/48/2/48_23047/_html/-char/en

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https://www.jstage.jst.go.jp/article/csf/48/2/48_23047/_html/-char/en

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