Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning.

Restriction enzyme body doubles and PCR cloning: on the general use of type IIs restriction enzymes for cloning.

The procedure described here allows the cloning of PCR fragments containing a recognition site of the restriction endonuclease (Type IIP) used for cloning in the sequence of the insert. A Type IIS endonuclease--a Body Double of the Type IIP enzyme--is used to generate the same protruding palindrome....

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Main Authors: Eszter Tóth, Krisztina Huszár, Petra Bencsura, Péter István Kulcsár, Barbara Vodicska, Antal Nyeste, Zsombor Welker, Szilvia Tóth, Ervin Welker
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618593/pdf/?tool=EBI

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https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24618593/pdf/?tool=EBI

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