Optimized proximity ligation assay (PLA) for detection of RNA-protein complex interactions in cell lines

Optimized proximity ligation assay (PLA) for detection of RNA-protein complex interactions in cell lines

Summary: Conventional proximity ligation assay (PLA) suffers from target specificity issues that curtail their accuracy on interpreting proximal interactions in cell biology. Here, we present a reliable and sensitive approach by including a fluorochrome-labeled mRNA fragment along with biotin-labele...

Full description

Bibliographic Details
Main Authors: Jasmine George, Sonam Mittal, Ishaque P. Kadamberi, Sunila Pradeep, Pradeep Chaluvally-Raghavan
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cancer
Cell Biology
Cell-based Assays
Microscopy
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722002209

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722002209

Similar Items