Protocol for using serial two-photon tomography to map cell types and cerebrovasculature at single-cell resolution in the whole adult mouse brain

Protocol for using serial two-photon tomography to map cell types and cerebrovasculature at single-cell resolution in the whole adult mouse brain

Summary: Here, we present a protocol using serial two-photon tomography (STPT) to quantitatively map genetically defined cell types and cerebrovasculature at single-cell resolution across the entire adult mouse brain. We describe the preparation of brain tissue and sample embedding for cell type and...

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Bibliographic Details
Main Authors: Josephine K. Liwang, Hannah C. Bennett, Hyun-Jae Pi, Yongsoo Kim
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Bioinformatics
Single Cell
Health Sciences
Microscopy
Neuroscience
Computer Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000060

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http://www.sciencedirect.com/science/article/pii/S2666166723000060

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