Gibson assembly interposition improves amplification efficiency of long DNA and multifragment overlap extension PCR
For difficult overlap extension PCR, a Gibson assembly process was inserted between the two PCR rounds to facilitate the formation of complete gene templates at a moderate temperature. That is, after amplifying each DNA fragment, they were preluded by a Gibson assembly process in equal proportion. T...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Future Science Ltd
2023-06-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0012 |