Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates

Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates

Rhamnogalacturonan lyase (RGL) cleaves backbone α-1,4 glycosidic bonds between L-rhamnose and D-galacturonic acid residues in type I rhamnogalacturonan (RG-I) by β-elimination to generate RG oligosaccharides with various degrees of polymerization. Here, we cloned, expressed, purified and biochemical...

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Bibliographic Details
Main Authors: Weiyang Wang, Yibing Wang, Haoting Yi, Yang Liu, Guojing Zhang, Le Zhang, Kevin H. Mayo, Ye Yuan, Yifa Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Microbiology
Subjects:
rhamnogalacturonan lyase
polysaccharide lyase family 11
Bacteroides ovatus
type I rhamnogalacturonan
rhamnogalacturonan oligosaccharides
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.799875/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2021.799875/full

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