Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses
Summary: Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722008851 |
| _version_ | 1828068354774007808 |
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| author | Rhea Vinchhi Chinmaya Jena Nishad Matange |
| author_facet | Rhea Vinchhi Chinmaya Jena Nishad Matange |
| author_sort | Rhea Vinchhi |
| collection | DOAJ |
| description | Summary: Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium Escherichia coli that can be easily adapted to answer questions related to evolution and genetics of AMR in diverse bacteria. Key issues to consider when designing ALE experiments as well as some downstream mutation mapping analyses are described.For complete details on the use and execution of this protocol, please refer to Patel and Matange (2021)1 and Matange et al. (2019).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T00:01:29Z |
| format | Article |
| id | doaj.art-be61437bbe924c968882de9c70e04108 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T00:01:29Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-be61437bbe924c968882de9c70e041082023-01-10T04:06:13ZengElsevierSTAR Protocols2666-16672023-03-0141102005Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analysesRhea Vinchhi0Chinmaya Jena1Nishad Matange2Department of Biology, Indian Institute of Science Education and Research, Pune, Maharashtra 411008, IndiaDepartment of Biology, Indian Institute of Science Education and Research, Pune, Maharashtra 411008, IndiaDepartment of Biology, Indian Institute of Science Education and Research, Pune, Maharashtra 411008, India; Corresponding authorSummary: Adaptive laboratory evolution (ALE) of bacteria has the potential to provide many insights like revealing novel mechanisms of resistance and elucidating the impact of drug combinations and concentrations on AMR evolution. Here, we describe a step-by-step ALE protocol for the model bacterium Escherichia coli that can be easily adapted to answer questions related to evolution and genetics of AMR in diverse bacteria. Key issues to consider when designing ALE experiments as well as some downstream mutation mapping analyses are described.For complete details on the use and execution of this protocol, please refer to Patel and Matange (2021)1 and Matange et al. (2019).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722008851GeneticsSequencingMicrobiologyModel OrganismsEvolutionary biology |
| spellingShingle | Rhea Vinchhi Chinmaya Jena Nishad Matange Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses STAR Protocols Genetics Sequencing Microbiology Model Organisms Evolutionary biology |
| title | Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses |
| title_full | Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses |
| title_fullStr | Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses |
| title_full_unstemmed | Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses |
| title_short | Adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses |
| title_sort | adaptive laboratory evolution of antimicrobial resistance in bacteria for genetic and phenotypic analyses |
| topic | Genetics Sequencing Microbiology Model Organisms Evolutionary biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722008851 |
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