RNA-seq validation: software for selection of reference and variable candidate genes for RT-qPCR

RNA-seq validation: software for selection of reference and variable candidate genes for RT-qPCR

Abstract Background Real-time quantitative PCR (RT-qPCR) is one of the most widely used gene expression analyses for validating RNA-seq data. This technique requires reference genes that are stable and highly expressed, at least across the different biological conditions present in the transcriptome...

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Bibliographic Details
Main Authors: Márcio Wilson Dias de Brito, Stephanie Serafim de Carvalho, Maria Beatriz dos Santos Mota, Rafael Dias Mesquita
Format: Article
Language:English
Published: BMC 2024-07-01
Series:BMC Genomics
Subjects:
Gene expression
Reference genes
Transcriptome validation
Aedes aegypti
RNA-seq
RT-qPCR
Online Access:https://doi.org/10.1186/s12864-024-10511-y

Internet

https://doi.org/10.1186/s12864-024-10511-y

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