Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome
Because of a loss-of-function mutation in the GGTA1 gene, humans are unable to synthetize α1,3-Galactose (Gal) decorated glycans and develop high levels of circulating anti-α1,3-Galactose antibodies (anti-Gal Abs). Anti-Gal Abs have been identified as a major obstacle of organ xenotransplantation an...
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| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.03000/full |
| _version_ | 1819109001561899008 |
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| author | Emmanuel Montassier Emmanuel Montassier Emmanuel Montassier Gabriel A. Al-Ghalith Camille Mathé Camille Mathé Quentin Le Bastard Quentin Le Bastard Venceslas Douillard Venceslas Douillard Venceslas Douillard Abel Garnier Abel Garnier Abel Garnier Rémi Guimon Rémi Guimon Rémi Guimon Bastien Raimondeau Bastien Raimondeau Yann Touchefeu Yann Touchefeu Emilie Duchalais Emilie Duchalais Nicolas Vince Nicolas Vince Sophie Limou Sophie Limou Pierre-Antoine Gourraud Pierre-Antoine Gourraud Pierre-Antoine Gourraud David A. Laplaud David A. Laplaud Arnaud B. Nicot Arnaud B. Nicot Jean-Paul Soulillou Jean-Paul Soulillou Laureline Berthelot Laureline Berthelot |
| author_facet | Emmanuel Montassier Emmanuel Montassier Emmanuel Montassier Gabriel A. Al-Ghalith Camille Mathé Camille Mathé Quentin Le Bastard Quentin Le Bastard Venceslas Douillard Venceslas Douillard Venceslas Douillard Abel Garnier Abel Garnier Abel Garnier Rémi Guimon Rémi Guimon Rémi Guimon Bastien Raimondeau Bastien Raimondeau Yann Touchefeu Yann Touchefeu Emilie Duchalais Emilie Duchalais Nicolas Vince Nicolas Vince Sophie Limou Sophie Limou Pierre-Antoine Gourraud Pierre-Antoine Gourraud Pierre-Antoine Gourraud David A. Laplaud David A. Laplaud Arnaud B. Nicot Arnaud B. Nicot Jean-Paul Soulillou Jean-Paul Soulillou Laureline Berthelot Laureline Berthelot |
| author_sort | Emmanuel Montassier |
| collection | DOAJ |
| description | Because of a loss-of-function mutation in the GGTA1 gene, humans are unable to synthetize α1,3-Galactose (Gal) decorated glycans and develop high levels of circulating anti-α1,3-Galactose antibodies (anti-Gal Abs). Anti-Gal Abs have been identified as a major obstacle of organ xenotransplantation and play a role in several host-pathogen relationships including potential susceptibility to infection. Anti-Gal Abs are supposed to stem from immunization against the gut microbiota, an assumption derived from the observation that some pathogens display α1,3-Gal and that antibiotic treatment decreases the level of anti-Gal. However, there is little information to date concerning the microorganisms producing α1,3-Gal in the human gut microbiome. Here, available α1,3-Galactosyltransferase (GT) gene sequences from gut bacteria were selectively quantified for the first time in the gut microbiome shotgun sequences of 163 adult individuals from three published population-based metagenomics analyses. We showed that most of the gut microbiome of adult individuals contained a small set of bacteria bearing α1,3-GT genes. These bacteria belong mainly to the Enterobacteriaceae family, including Escherichia coli, but also to Pasteurellaceae genera, Haemophilus influenza and Lactobacillus species. α1,3-Gal antigens and α1,3-GT activity were detected in healthy stools of individuals exhibiting α1,3-GT bacterial gene sequences in their shotgun data. |
| first_indexed | 2024-12-22T03:18:53Z |
| format | Article |
| id | doaj.art-be86596bdcbd4dbfb32b4645206d631f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-22T03:18:53Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-be86596bdcbd4dbfb32b4645206d631f2022-12-21T18:40:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-01-011010.3389/fimmu.2019.03000479459Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut MicrobiomeEmmanuel Montassier0Emmanuel Montassier1Emmanuel Montassier2Gabriel A. Al-Ghalith3Camille Mathé4Camille Mathé5Quentin Le Bastard6Quentin Le Bastard7Venceslas Douillard8Venceslas Douillard9Venceslas Douillard10Abel Garnier11Abel Garnier12Abel Garnier13Rémi Guimon14Rémi Guimon15Rémi Guimon16Bastien Raimondeau17Bastien Raimondeau18Yann Touchefeu19Yann Touchefeu20Emilie Duchalais21Emilie Duchalais22Nicolas Vince23Nicolas Vince24Sophie Limou25Sophie Limou26Pierre-Antoine Gourraud27Pierre-Antoine Gourraud28Pierre-Antoine Gourraud29David A. Laplaud30David A. Laplaud31Arnaud B. Nicot32Arnaud B. Nicot33Jean-Paul Soulillou34Jean-Paul Soulillou35Laureline Berthelot36Laureline Berthelot37Microbiota Hosts Antibiotics and bacterial Resistances (MiHAR), Université de Nantes, Nantes, FranceLaboratoire EA3826 Thérapeutiques cliniques et expérimentales des infections IRS2 Nantes Biotech, Université de Nantes, Nantes, FranceDepartment of Emergency Medicine, CHU de Nantes, Nantes, FranceBioinformatics and Computational Biology, University of Minnesota, Minneapolis, MN, United StatesCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceMicrobiota Hosts Antibiotics and bacterial Resistances (MiHAR), Université de Nantes, Nantes, FranceDepartment of Emergency Medicine, CHU de Nantes, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCHU de Nantes, CIC 1413, Pôle Hospitalo-Universitaire 11 Santé Publique, Clinique des données, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCHU de Nantes, CIC 1413, Pôle Hospitalo-Universitaire 11 Santé Publique, Clinique des données, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCHU de Nantes, CIC 1413, Pôle Hospitalo-Universitaire 11 Santé Publique, Clinique des données, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceInstitut des Maladies de l'Appareil Digestif, CHU Nantes, Nantes, FranceINSERM U1235, Nantes, FranceInstitut des Maladies de l'Appareil Digestif, CHU Nantes, Nantes, FranceINSERM U1235, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCHU de Nantes, CIC 1413, Pôle Hospitalo-Universitaire 11 Santé Publique, Clinique des données, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, France0Neurology department, CIC Neurology, CHU de Nantes, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceCentre de Recherche en Transplantation et Immunologie UMR 1064, INSERM, Université de Nantes, Nantes, FranceInstitut de Transplantation Urologie Néphrologie (ITUN), CHU de Nantes, Nantes, FranceBecause of a loss-of-function mutation in the GGTA1 gene, humans are unable to synthetize α1,3-Galactose (Gal) decorated glycans and develop high levels of circulating anti-α1,3-Galactose antibodies (anti-Gal Abs). Anti-Gal Abs have been identified as a major obstacle of organ xenotransplantation and play a role in several host-pathogen relationships including potential susceptibility to infection. Anti-Gal Abs are supposed to stem from immunization against the gut microbiota, an assumption derived from the observation that some pathogens display α1,3-Gal and that antibiotic treatment decreases the level of anti-Gal. However, there is little information to date concerning the microorganisms producing α1,3-Gal in the human gut microbiome. Here, available α1,3-Galactosyltransferase (GT) gene sequences from gut bacteria were selectively quantified for the first time in the gut microbiome shotgun sequences of 163 adult individuals from three published population-based metagenomics analyses. We showed that most of the gut microbiome of adult individuals contained a small set of bacteria bearing α1,3-GT genes. These bacteria belong mainly to the Enterobacteriaceae family, including Escherichia coli, but also to Pasteurellaceae genera, Haemophilus influenza and Lactobacillus species. α1,3-Gal antigens and α1,3-GT activity were detected in healthy stools of individuals exhibiting α1,3-GT bacterial gene sequences in their shotgun data.https://www.frontiersin.org/article/10.3389/fimmu.2019.03000/fullintestinal microbiomeα1,3-Galactosyltransferaseα1,3-Gal antigenshotgun dataenterobacteriaceae |
| spellingShingle | Emmanuel Montassier Emmanuel Montassier Emmanuel Montassier Gabriel A. Al-Ghalith Camille Mathé Camille Mathé Quentin Le Bastard Quentin Le Bastard Venceslas Douillard Venceslas Douillard Venceslas Douillard Abel Garnier Abel Garnier Abel Garnier Rémi Guimon Rémi Guimon Rémi Guimon Bastien Raimondeau Bastien Raimondeau Yann Touchefeu Yann Touchefeu Emilie Duchalais Emilie Duchalais Nicolas Vince Nicolas Vince Sophie Limou Sophie Limou Pierre-Antoine Gourraud Pierre-Antoine Gourraud Pierre-Antoine Gourraud David A. Laplaud David A. Laplaud Arnaud B. Nicot Arnaud B. Nicot Jean-Paul Soulillou Jean-Paul Soulillou Laureline Berthelot Laureline Berthelot Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome Frontiers in Immunology intestinal microbiome α1,3-Galactosyltransferase α1,3-Gal antigen shotgun data enterobacteriaceae |
| title | Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome |
| title_full | Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome |
| title_fullStr | Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome |
| title_full_unstemmed | Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome |
| title_short | Distribution of Bacterial α1,3-Galactosyltransferase Genes in the Human Gut Microbiome |
| title_sort | distribution of bacterial α1 3 galactosyltransferase genes in the human gut microbiome |
| topic | intestinal microbiome α1,3-Galactosyltransferase α1,3-Gal antigen shotgun data enterobacteriaceae |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.03000/full |
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