Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy
ABSTRACT Purpose: To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models. Methods: The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-02-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001100206&tlng=en |
| _version_ | 1819277795407167488 |
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| author | Hui Zhang Shu Jia Lianyu Jin Jianzuo Yao MB Zhihong Shen Jingyi Wu Xiaokun Yao Danwei Chen Congcong Zhang Shufang Yu Ningwei Zhu Lexiao Jin Xiaomin Yao |
| author_facet | Hui Zhang Shu Jia Lianyu Jin Jianzuo Yao MB Zhihong Shen Jingyi Wu Xiaokun Yao Danwei Chen Congcong Zhang Shufang Yu Ningwei Zhu Lexiao Jin Xiaomin Yao |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | ABSTRACT Purpose: To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models. Methods: The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered an equal volume of saline daily for five weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic histopathological examinations, and Masson staining were performed to evaluate liver injury. Liver intercellular adhesion molecule-1 (ICAM-1) and P-selectin were evaluated by immunohistochemistry. Hepatocellular apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Protein expression levels of autophagy markers were measured using Western blot analysis. Results: Gynura segetum was found to significantly induce liver injury compared with control mice, as evidenced by the increase of serum transaminases, a decrease in triglyceride levels, and histopathological changes in mice. Gynura segetum remarkably induced hepatocellular apoptosis and upregulated the expressions of ICAM-1 and P-selectin and also downregulated the protein expression levels of LC3, Atg12 and cytoplasmic polyadenylation element binding protein. Conclusions: Our results suggested that G. segetum induced liver injury with HSOS, and it was partly due to its ability to impair the autophagy pathway. |
| first_indexed | 2024-12-24T00:01:47Z |
| format | Article |
| id | doaj.art-be91eaf3599c4cb498f850b91f5e1508 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-12-24T00:01:47Z |
| publishDate | 2022-02-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-be91eaf3599c4cb498f850b91f5e15082022-12-21T17:25:07ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-02-01361110.1590/acb361104Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagyHui Zhanghttps://orcid.org/0000-0001-8031-6424Shu Jiahttps://orcid.org/0000-0001-8208-1315Lianyu Jinhttps://orcid.org/0000-0003-0969-9519Jianzuo Yao MBhttps://orcid.org/0000-0001-8642-4194Zhihong Shenhttps://orcid.org/0000-0002-3975-856XJingyi Wuhttps://orcid.org/0000-0002-7221-6658Xiaokun Yaohttps://orcid.org/0000-0003-3481-6174Danwei Chenhttps://orcid.org/0000-0001-5326-645XCongcong Zhanghttps://orcid.org/0000-0002-3156-7574Shufang Yuhttps://orcid.org/0000-0002-3347-4638Ningwei Zhuhttps://orcid.org/0000-0002-6115-8990Lexiao Jinhttps://orcid.org/0000-0002-1177-6416Xiaomin Yaohttps://orcid.org/0000-0002-3467-0868ABSTRACT Purpose: To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models. Methods: The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered an equal volume of saline daily for five weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic histopathological examinations, and Masson staining were performed to evaluate liver injury. Liver intercellular adhesion molecule-1 (ICAM-1) and P-selectin were evaluated by immunohistochemistry. Hepatocellular apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Protein expression levels of autophagy markers were measured using Western blot analysis. Results: Gynura segetum was found to significantly induce liver injury compared with control mice, as evidenced by the increase of serum transaminases, a decrease in triglyceride levels, and histopathological changes in mice. Gynura segetum remarkably induced hepatocellular apoptosis and upregulated the expressions of ICAM-1 and P-selectin and also downregulated the protein expression levels of LC3, Atg12 and cytoplasmic polyadenylation element binding protein. Conclusions: Our results suggested that G. segetum induced liver injury with HSOS, and it was partly due to its ability to impair the autophagy pathway.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001100206&tlng=enHepatic Veno-Occlusive DiseasePyrrolizidine AlkaloidsLiverAutophagyMice |
| spellingShingle | Hui Zhang Shu Jia Lianyu Jin Jianzuo Yao MB Zhihong Shen Jingyi Wu Xiaokun Yao Danwei Chen Congcong Zhang Shufang Yu Ningwei Zhu Lexiao Jin Xiaomin Yao Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy Acta Cirúrgica Brasileira Hepatic Veno-Occlusive Disease Pyrrolizidine Alkaloids Liver Autophagy Mice |
| title | Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy |
| title_full | Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy |
| title_fullStr | Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy |
| title_full_unstemmed | Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy |
| title_short | Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy |
| title_sort | gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy |
| topic | Hepatic Veno-Occlusive Disease Pyrrolizidine Alkaloids Liver Autophagy Mice |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001100206&tlng=en |
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