FACS-based purification of <it>Arabidopsis</it> microspores, sperm cells and vegetative nuclei

FACS-based purification of <it>Arabidopsis</it> microspores, sperm cells and vegetative nuclei

<p>Abstract</p> <p>Background</p> <p>The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes a second mitosis to ori...

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Bibliographic Details
Main Authors: Borges Filipe, Gardner Rui, Lopes Telma, Calarco Joseph P, Boavida Leonor C, Slotkin R, Martienssen Robert A, Becker Jörg D
Format: Article
Language:English
Published: BMC 2012-10-01
Series:Plant Methods
Subjects:
Pollen
Microspore
Sperm cell
Vegetative nucleus
Plant germline
<it>Arabidopsis</it>
Fluorescence-activated cell sorting
Online Access:http://www.plantmethods.com/content/8/1/44

Internet

http://www.plantmethods.com/content/8/1/44

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