Alteration of Substrate Specificity and Transglucosylation Activity of GH13_31 α-Glucosidase from <i>Bacillus</i> sp. AHU2216 through Site-Directed Mutagenesis of Asn258 on β→α Loop 5

Alteration of Substrate Specificity and Transglucosylation Activity of GH13_31 α-Glucosidase from <i>Bacillus</i> sp. AHU2216 through Site-Directed Mutagenesis of Asn258 on β→α Loop 5

α-Glucosidase catalyzes the hydrolysis of α-<span style="font-variant: small-caps;">d</span>-glucosides and transglucosylation. <i>Bacillus</i> sp. AHU2216 α-glucosidase (BspAG13_31A), belonging to the glycoside hydrolase family 13 subfamily 31, specifically cleaves...

Full description

Bibliographic Details
Main Authors: Waraporn Auiewiriyanukul, Wataru Saburi, Tomoya Ota, Jian Yu, Koji Kato, Min Yao, Haruhide Mori
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Molecules
Subjects:
α-glucosidase
glycoside hydrolase family 13
transglycosylation
substrate specificity
site-directed mutagenesis
X-ray crystallography
Online Access:https://www.mdpi.com/1420-3049/28/7/3109

Internet

https://www.mdpi.com/1420-3049/28/7/3109

Similar Items