Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane

Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane

Although second harmonic generation (SHG) microscopy provides unique imaging advantages for voltage imaging and other biological applications, genetically-encoded SHG chromophores remain relatively unexplored. SHG only arises from non-centrosymmetric media, so an anisotropic arrangement of chromopho...

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Bibliographic Details
Main Authors: Yuka eJinno, Keiko eShoda, Emiliano eRial-Verde, Rafael eYuste, Miyawaki eAtsushi, Hidekazu eTsutsui
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-11-01
Series:Frontiers in Molecular Neuroscience
Subjects:
Mutagenesis
electrostatic potential
second harmonic generation
fluoresence protein
Kras4B
Online Access:http://journal.frontiersin.org/Journal/10.3389/fnmol.2014.00093/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fnmol.2014.00093/full

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