Efficient and Cost-Effective Single Nucleotide Polymorphism Detection with Different Fluorescent Applications
Three methods—5′ nuclease assay with TaqMan®, minisequencing, and oligonucleotide ligation assay (OLA)—were compared to detect five single nucleotide polymorphisms (SNPs) in three separate genes. Each method had advantages and disadvantages. The 5′ nuclease assay was the fastest and required only a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01314rr03 |