The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans
Understanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward yeast genetics. The origin recognition complexes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/13/1/13 |
| _version_ | 1797344576647725056 |
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| author | Bik-Kwoon Tye Yuanliang Zhai |
| author_facet | Bik-Kwoon Tye Yuanliang Zhai |
| author_sort | Bik-Kwoon Tye |
| collection | DOAJ |
| description | Understanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward yeast genetics. The origin recognition complexes (ORC) that load the MCM hexamers at replication origins were purified from yeast extracts. We have reached an age where high-resolution cryoEM structures of yeast and human replication complexes can be compared side-by-side. Their similarities and differences are converging as alternative strategies that may deviate in detail but are shared by both species. |
| first_indexed | 2024-03-08T11:05:40Z |
| format | Article |
| id | doaj.art-bee22c1d798f4d7a90571c1add4367d4 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-08T11:05:40Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-bee22c1d798f4d7a90571c1add4367d42024-01-26T15:06:41ZengMDPI AGBiology2079-77372023-12-011311310.3390/biology13010013The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and HumansBik-Kwoon Tye0Yuanliang Zhai1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USASchool of Biological Sciences, The University of Hong Kong, Hong Kong, ChinaUnderstanding human DNA replication through the study of yeast has been an extremely fruitful journey. The minichromosome maintenance (MCM) 2–7 genes that encode the catalytic core of the eukaryotic replisome were initially identified through forward yeast genetics. The origin recognition complexes (ORC) that load the MCM hexamers at replication origins were purified from yeast extracts. We have reached an age where high-resolution cryoEM structures of yeast and human replication complexes can be compared side-by-side. Their similarities and differences are converging as alternative strategies that may deviate in detail but are shared by both species.https://www.mdpi.com/2079-7737/13/1/13origin selectionreplication initiationeukaryotic DNA replicationcryoEM structuresMCM hexamersorigin recognition complex |
| spellingShingle | Bik-Kwoon Tye Yuanliang Zhai The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans Biology origin selection replication initiation eukaryotic DNA replication cryoEM structures MCM hexamers origin recognition complex |
| title | The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans |
| title_full | The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans |
| title_fullStr | The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans |
| title_full_unstemmed | The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans |
| title_short | The Origin Recognition Complex: From Origin Selection to Replication Licensing in Yeast and Humans |
| title_sort | origin recognition complex from origin selection to replication licensing in yeast and humans |
| topic | origin selection replication initiation eukaryotic DNA replication cryoEM structures MCM hexamers origin recognition complex |
| url | https://www.mdpi.com/2079-7737/13/1/13 |
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