Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview
Aortic valve stenosis secondary to aortic valve calcification is the most common valve disease in the Western world. Calcification is a result of pathological proliferation and osteogenic differentiation of resident valve interstitial cells. To develop non-surgical treatments, the molecular and cell...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.835825/full |
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| author | Maria Bogdanova Arsenii Zabirnyk Arsenii Zabirnyk Anna Malashicheva Daria Semenova John-Peder Escobar Kvitting Mari-Liis Kaljusto Maria del Mar Perez Anna Kostareva Anna Kostareva Kåre-Olav Stensløkken Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Arkady Rutkovskiy Arkady Rutkovskiy Jarle Vaage Jarle Vaage Jarle Vaage |
| author_facet | Maria Bogdanova Arsenii Zabirnyk Arsenii Zabirnyk Anna Malashicheva Daria Semenova John-Peder Escobar Kvitting Mari-Liis Kaljusto Maria del Mar Perez Anna Kostareva Anna Kostareva Kåre-Olav Stensløkken Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Arkady Rutkovskiy Arkady Rutkovskiy Jarle Vaage Jarle Vaage Jarle Vaage |
| author_sort | Maria Bogdanova |
| collection | DOAJ |
| description | Aortic valve stenosis secondary to aortic valve calcification is the most common valve disease in the Western world. Calcification is a result of pathological proliferation and osteogenic differentiation of resident valve interstitial cells. To develop non-surgical treatments, the molecular and cellular mechanisms of pathological calcification must be revealed. In the current overview, we present methods for evaluation of calcification in different ex vivo, in vitro and in vivo situations including imaging in patients. The latter include echocardiography, scanning with computed tomography and magnetic resonance imaging. Particular emphasis is on translational studies of calcific aortic valve stenosis with a special focus on cell culture using human primary cell cultures. Such models are widely used and suitable for screening of drugs against calcification. Animal models are presented, but there is no animal model that faithfully mimics human calcific aortic valve disease. A model of experimentally induced calcification in whole porcine aortic valve leaflets ex vivo is also included. Finally, miscellaneous methods and aspects of aortic valve calcification, such as, for instance, biomarkers are presented. |
| first_indexed | 2024-04-13T22:27:10Z |
| format | Article |
| id | doaj.art-bee968d2f14e495b851a83949d91c8bb |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T22:27:10Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-bee968d2f14e495b851a83949d91c8bb2022-12-22T02:27:02ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-06-011310.3389/fphar.2022.835825835825Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An OverviewMaria Bogdanova0Arsenii Zabirnyk1Arsenii Zabirnyk2Anna Malashicheva3Daria Semenova4John-Peder Escobar Kvitting5Mari-Liis Kaljusto6Maria del Mar Perez7Anna Kostareva8Anna Kostareva9Kåre-Olav Stensløkken10Gareth J Sullivan11Gareth J Sullivan12Gareth J Sullivan13Gareth J Sullivan14Gareth J Sullivan15Arkady Rutkovskiy16Arkady Rutkovskiy17Jarle Vaage18Jarle Vaage19Jarle Vaage20Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Research and Development, Division of Emergencies and Critical Care, Oslo University Hospital, Oslo, NorwayInstitute of Cytology, Russian Academy of Sciences, Saint Petersburg, RussiaInstitute of Cytology, Russian Academy of Sciences, Saint Petersburg, RussiaDepartment of Cardiothoracic Surgery, Oslo University Hospital, Oslo, NorwayDepartment of Cardiothoracic Surgery, Oslo University Hospital, Oslo, NorwaySanifit Therapeutics, Palma de Mallorca, SpainAlmazov National Medical Research Centre, Saint Petersburg, RussiaDepartment of Woman and Children Health, Karolinska Institute, Stockholm, SwedenDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayNorwegian Center for Stem Cell Research, Oslo University Hospital and University of Oslo, Oslo, NorwayInstitute of Immunology, Oslo University Hospital, Oslo, Norway0Hybrid Technology Hub - Centre of Excellence, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway1Department of Pediatric Research, Oslo University Hospital, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway2Department of Pulmonary Diseases, Oslo University Hospital, Oslo, NorwayDepartment of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Oslo, NorwayDepartment of Research and Development, Division of Emergencies and Critical Care, Oslo University Hospital, Oslo, Norway3Institute of Clinical Medicine, University of Oslo, Oslo, NorwayAortic valve stenosis secondary to aortic valve calcification is the most common valve disease in the Western world. Calcification is a result of pathological proliferation and osteogenic differentiation of resident valve interstitial cells. To develop non-surgical treatments, the molecular and cellular mechanisms of pathological calcification must be revealed. In the current overview, we present methods for evaluation of calcification in different ex vivo, in vitro and in vivo situations including imaging in patients. The latter include echocardiography, scanning with computed tomography and magnetic resonance imaging. Particular emphasis is on translational studies of calcific aortic valve stenosis with a special focus on cell culture using human primary cell cultures. Such models are widely used and suitable for screening of drugs against calcification. Animal models are presented, but there is no animal model that faithfully mimics human calcific aortic valve disease. A model of experimentally induced calcification in whole porcine aortic valve leaflets ex vivo is also included. Finally, miscellaneous methods and aspects of aortic valve calcification, such as, for instance, biomarkers are presented.https://www.frontiersin.org/articles/10.3389/fphar.2022.835825/fullaortic valveinterstitial cellsendothelial cellscalcificationanimal modelscalcified aortic valve disease |
| spellingShingle | Maria Bogdanova Arsenii Zabirnyk Arsenii Zabirnyk Anna Malashicheva Daria Semenova John-Peder Escobar Kvitting Mari-Liis Kaljusto Maria del Mar Perez Anna Kostareva Anna Kostareva Kåre-Olav Stensløkken Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Gareth J Sullivan Arkady Rutkovskiy Arkady Rutkovskiy Jarle Vaage Jarle Vaage Jarle Vaage Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview Frontiers in Pharmacology aortic valve interstitial cells endothelial cells calcification animal models calcified aortic valve disease |
| title | Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview |
| title_full | Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview |
| title_fullStr | Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview |
| title_full_unstemmed | Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview |
| title_short | Models and Techniques to Study Aortic Valve Calcification in Vitro, ex Vivo and in Vivo. An Overview |
| title_sort | models and techniques to study aortic valve calcification in vitro ex vivo and in vivo an overview |
| topic | aortic valve interstitial cells endothelial cells calcification animal models calcified aortic valve disease |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.835825/full |
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