Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation
Background: Efficient induction of fetal hemoglobin (HbF) is considered as an effective therapeutic approach in beta thalassemia. HbF inducer agents can induce the expression of γ-globin gene and produce high levels of HbF via different epigenetic and molecular mechanisms. Thalidomide and sodium but...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-04-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/368 |
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| author | Mohammad Ali Jalali Far Ali Dehghani Fard Saiedeh Hajizamani Majid Mossahebi-Mohammadi Hamid Yaghooti Najmaldin Saki |
| author_facet | Mohammad Ali Jalali Far Ali Dehghani Fard Saiedeh Hajizamani Majid Mossahebi-Mohammadi Hamid Yaghooti Najmaldin Saki |
| author_sort | Mohammad Ali Jalali Far |
| collection | DOAJ |
| description | Background: Efficient induction of fetal hemoglobin (HbF) is considered as an effective therapeutic approach in beta thalassemia. HbF inducer agents can induce the expression of γ-globin gene and produce high levels of HbF via different epigenetic and molecular mechanisms. Thalidomide and sodium butyrate are known as HbF inducer drugs.
Material and methods: CD133+ stem cells were isolated from umbilical cord blood of a newborn with minor β-thalassemia in order to evaluate the effects of these two drugs on the in vitro expression of GATA-1 and EKLF genes as erythroid transcription factors. CD133+ stem cells were expanded and differentiated into erythroid lineage, and then treated with thalidomide and sodium butyrate and finally analyzed by quantitative real-time PCR. Statistical analysis was performed using student’s t-test by SPSS software.
Results: Thalidomide and sodium butyrate increased GATA-1 and EKLF gene expression, compared to the non-treated control (P<0.05).
Conclusion: Thalidomide was more efficient than sodium butyrate in augmenting expression of GATA-1 and EKLF genes. It seems that GATA-1 and EKLF have crucial roles in the efficient induction of HbF by thalidomide. |
| first_indexed | 2024-03-12T10:46:54Z |
| format | Article |
| id | doaj.art-bef6c0aa43ef4460b0f812e52c417b23 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T10:46:54Z |
| publishDate | 2016-04-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-bef6c0aa43ef4460b0f812e52c417b232023-09-02T07:27:08ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072016-04-01101Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutationMohammad Ali Jalali Far0Ali Dehghani Fard1Saiedeh Hajizamani2Majid Mossahebi-Mohammadi3Hamid Yaghooti4Najmaldin Saki5Health research institute, Research Center of Thalassemia & Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranSarem Cell Research Center-SCRC, Sarem Women’s Hospital, Tehran, IranDiagnosticLaboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Medical Laboratory Sciences, School of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranHealth research institute, Research Center of Thalassemia & Hemoglobinopathy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Efficient induction of fetal hemoglobin (HbF) is considered as an effective therapeutic approach in beta thalassemia. HbF inducer agents can induce the expression of γ-globin gene and produce high levels of HbF via different epigenetic and molecular mechanisms. Thalidomide and sodium butyrate are known as HbF inducer drugs. Material and methods: CD133+ stem cells were isolated from umbilical cord blood of a newborn with minor β-thalassemia in order to evaluate the effects of these two drugs on the in vitro expression of GATA-1 and EKLF genes as erythroid transcription factors. CD133+ stem cells were expanded and differentiated into erythroid lineage, and then treated with thalidomide and sodium butyrate and finally analyzed by quantitative real-time PCR. Statistical analysis was performed using student’s t-test by SPSS software. Results: Thalidomide and sodium butyrate increased GATA-1 and EKLF gene expression, compared to the non-treated control (P<0.05). Conclusion: Thalidomide was more efficient than sodium butyrate in augmenting expression of GATA-1 and EKLF genes. It seems that GATA-1 and EKLF have crucial roles in the efficient induction of HbF by thalidomide.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/368Fetal hemoglobinThalidomideSodium butyrateβ-thalassemia |
| spellingShingle | Mohammad Ali Jalali Far Ali Dehghani Fard Saiedeh Hajizamani Majid Mossahebi-Mohammadi Hamid Yaghooti Najmaldin Saki Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation International Journal of Hematology-Oncology and Stem Cell Research Fetal hemoglobin Thalidomide Sodium butyrate β-thalassemia |
| title | Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation |
| title_full | Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation |
| title_fullStr | Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation |
| title_full_unstemmed | Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation |
| title_short | Thalidomide is more efficient than sodium butyrate in enhancing GATA-1 and EKLF gene expression in erythroid progenitors derived from HSCs with β-globin gene mutation |
| title_sort | thalidomide is more efficient than sodium butyrate in enhancing gata 1 and eklf gene expression in erythroid progenitors derived from hscs with β globin gene mutation |
| topic | Fetal hemoglobin Thalidomide Sodium butyrate β-thalassemia |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/368 |
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