An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian
SoxB transcription factors and histone deacetylases (HDACs) are each major players in the regulation of neurogenesis, but a functional link between them has not been previously demonstrated. Here, we show that SoxB2 and Hdac2 act together to regulate neurogenesis in the cnidarian Hydractinia echinat...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717300554 |
| _version_ | 1818358133629648896 |
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| author | Hakima Flici Christine E. Schnitzler R. Cathriona Millane Graham Govinden Amy Houlihan Stephanie D. Boomkamp Sanbing Shen Andreas D. Baxevanis Uri Frank |
| author_facet | Hakima Flici Christine E. Schnitzler R. Cathriona Millane Graham Govinden Amy Houlihan Stephanie D. Boomkamp Sanbing Shen Andreas D. Baxevanis Uri Frank |
| author_sort | Hakima Flici |
| collection | DOAJ |
| description | SoxB transcription factors and histone deacetylases (HDACs) are each major players in the regulation of neurogenesis, but a functional link between them has not been previously demonstrated. Here, we show that SoxB2 and Hdac2 act together to regulate neurogenesis in the cnidarian Hydractinia echinata during tissue homeostasis and head regeneration. We find that misexpression of SoxB genes modifies the number of neural cells in all life stages and interferes with head regeneration. Hdac2 was co-expressed with SoxB2, and its downregulation phenocopied SoxB2 knockdown. We also show that SoxB2 and Hdac2 promote each other’s transcript levels, but Hdac2 counteracts this amplification cycle by deacetylating and destabilizing SoxB2 protein. Finally, we present evidence for conservation of these interactions in human neural progenitors. We hypothesize that crosstalk between SoxB transcription factors and Hdac2 is an ancient feature of metazoan neurogenesis and functions to stabilize the correct levels of these multifunctional proteins. |
| first_indexed | 2024-12-13T20:24:10Z |
| format | Article |
| id | doaj.art-bef769dbf914420fac71931a0f6f6ee3 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-13T20:24:10Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-bef769dbf914420fac71931a0f6f6ee32022-12-21T23:32:37ZengElsevierCell Reports2211-12472017-02-011861395140910.1016/j.celrep.2017.01.019An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a CnidarianHakima Flici0Christine E. Schnitzler1R. Cathriona Millane2Graham Govinden3Amy Houlihan4Stephanie D. Boomkamp5Sanbing Shen6Andreas D. Baxevanis7Uri Frank8Centre for Chromosome Biology (CCB), School of Natural Sciences, National University of Ireland, Galway H91 CF50, IrelandWhitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USACentre for Chromosome Biology (CCB), School of Natural Sciences, National University of Ireland, Galway H91 CF50, IrelandCentre for Chromosome Biology (CCB), School of Natural Sciences, National University of Ireland, Galway H91 CF50, IrelandCentre for Chromosome Biology (CCB), School of Natural Sciences, National University of Ireland, Galway H91 CF50, IrelandRegenerative Medicine Institute (REMEDI), National University of Ireland, Galway, Galway H91 CF50, IrelandRegenerative Medicine Institute (REMEDI), National University of Ireland, Galway, Galway H91 CF50, IrelandComputational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892-8002, USACentre for Chromosome Biology (CCB), School of Natural Sciences, National University of Ireland, Galway H91 CF50, IrelandSoxB transcription factors and histone deacetylases (HDACs) are each major players in the regulation of neurogenesis, but a functional link between them has not been previously demonstrated. Here, we show that SoxB2 and Hdac2 act together to regulate neurogenesis in the cnidarian Hydractinia echinata during tissue homeostasis and head regeneration. We find that misexpression of SoxB genes modifies the number of neural cells in all life stages and interferes with head regeneration. Hdac2 was co-expressed with SoxB2, and its downregulation phenocopied SoxB2 knockdown. We also show that SoxB2 and Hdac2 promote each other’s transcript levels, but Hdac2 counteracts this amplification cycle by deacetylating and destabilizing SoxB2 protein. Finally, we present evidence for conservation of these interactions in human neural progenitors. We hypothesize that crosstalk between SoxB transcription factors and Hdac2 is an ancient feature of metazoan neurogenesis and functions to stabilize the correct levels of these multifunctional proteins.http://www.sciencedirect.com/science/article/pii/S2211124717300554nervous systemregenerationtranscription factorSoxBhistone deacetylaseHdac2Hydractiniaevolutioncnidarianeurogenesis |
| spellingShingle | Hakima Flici Christine E. Schnitzler R. Cathriona Millane Graham Govinden Amy Houlihan Stephanie D. Boomkamp Sanbing Shen Andreas D. Baxevanis Uri Frank An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian Cell Reports nervous system regeneration transcription factor SoxB histone deacetylase Hdac2 Hydractinia evolution cnidaria neurogenesis |
| title | An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian |
| title_full | An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian |
| title_fullStr | An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian |
| title_full_unstemmed | An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian |
| title_short | An Evolutionarily Conserved SoxB-Hdac2 Crosstalk Regulates Neurogenesis in a Cnidarian |
| title_sort | evolutionarily conserved soxb hdac2 crosstalk regulates neurogenesis in a cnidarian |
| topic | nervous system regeneration transcription factor SoxB histone deacetylase Hdac2 Hydractinia evolution cnidaria neurogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717300554 |
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