Detection of soy in food from the Czech market using ELISA and PCR methods
Soy is considered an allergen under Regulation No. 1169/2011 of the European Parliament and of the Council, which mandates the labelling of soy allergen on food packaging. This study is focused on detecting soy in food products using two kinds of methods. The first method, enzyme-linked immunosorben...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2022-05-01
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| Series: | Czech Journal of Food Sciences |
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| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-202205-0006_detection-of-soy-in-food-from-the-czech-market-using-elisa-and-pcr-methods.php |
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| author | Kamila Novotná Kružíková Tereza Popelková Zuzana Tšponová Veronika Doubková Petra Doleželová Lucie Laichmannová |
| author_facet | Kamila Novotná Kružíková Tereza Popelková Zuzana Tšponová Veronika Doubková Petra Doleželová Lucie Laichmannová |
| author_sort | Kamila Novotná Kružíková |
| collection | DOAJ |
| description | Soy is considered an allergen under Regulation No. 1169/2011 of the European Parliament and of the Council, which mandates the labelling of soy allergen on food packaging. This study is focused on detecting soy in food products using two kinds of methods. The first method, enzyme-linked immunosorbent assay (ELISA), was performed using two commercial kits (Veratox for Soy Allergen; Neogen, USA). The second method, polymerase chain reaction (PCR), was carried out with two different deoxyribonucleic acid (DNA)-isolating kits and DNA isolation via the cetyltrimethylammonium bromide (CTAB) method. A total of 57 samples of food were tested, including 45 samples of animal origin and 12 samples of plant origin. The results were compared with information on the packaging. From the group of samples that contained soy according to the packaging (12 pieces), 9 samples were found to be positive by ELISA method, 10 samples by the CTAB method and 11 samples by GeneSpin. On the other hand, from the group of samples that should not contain soy according to the packaging (30 pieces), the presence of soy in 2 samples was detected by ELISA. No significant difference was found between the examined methods. Results show that the situation on the market is satisfactory and that only products declared as containing traces of soy appear to be problematic. |
| first_indexed | 2024-04-10T08:31:56Z |
| format | Article |
| id | doaj.art-bf119e902c8c4ffb949268771b787ff5 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:31:56Z |
| publishDate | 2022-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-bf119e902c8c4ffb949268771b787ff52023-02-23T03:28:51ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172022-05-0140537538210.17221/100/2021-CJFScjf-202205-0006Detection of soy in food from the Czech market using ELISA and PCR methodsKamila Novotná KružíkováTereza Popelková0Zuzana Tšponová1Veronika Doubková2Petra Doleželová3Lucie Laichmannová4Department of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech RepublicDepartment of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech RepublicDepartment of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech RepublicDepartment of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech RepublicDepartment of Animal Protection and Welfare and Veterinary Public Health, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, Brno, Czech RepublicSoy is considered an allergen under Regulation No. 1169/2011 of the European Parliament and of the Council, which mandates the labelling of soy allergen on food packaging. This study is focused on detecting soy in food products using two kinds of methods. The first method, enzyme-linked immunosorbent assay (ELISA), was performed using two commercial kits (Veratox for Soy Allergen; Neogen, USA). The second method, polymerase chain reaction (PCR), was carried out with two different deoxyribonucleic acid (DNA)-isolating kits and DNA isolation via the cetyltrimethylammonium bromide (CTAB) method. A total of 57 samples of food were tested, including 45 samples of animal origin and 12 samples of plant origin. The results were compared with information on the packaging. From the group of samples that contained soy according to the packaging (12 pieces), 9 samples were found to be positive by ELISA method, 10 samples by the CTAB method and 11 samples by GeneSpin. On the other hand, from the group of samples that should not contain soy according to the packaging (30 pieces), the presence of soy in 2 samples was detected by ELISA. No significant difference was found between the examined methods. Results show that the situation on the market is satisfactory and that only products declared as containing traces of soy appear to be problematic.https://cjfs.agriculturejournals.cz/artkey/cjf-202205-0006_detection-of-soy-in-food-from-the-czech-market-using-elisa-and-pcr-methods.phpallergenprocessed productproduct labellingregulation no. 1169/2011ctab method |
| spellingShingle | Kamila Novotná Kružíková Tereza Popelková Zuzana Tšponová Veronika Doubková Petra Doleželová Lucie Laichmannová Detection of soy in food from the Czech market using ELISA and PCR methods Czech Journal of Food Sciences allergen processed product product labelling regulation no. 1169/2011 ctab method |
| title | Detection of soy in food from the Czech market using ELISA and PCR methods |
| title_full | Detection of soy in food from the Czech market using ELISA and PCR methods |
| title_fullStr | Detection of soy in food from the Czech market using ELISA and PCR methods |
| title_full_unstemmed | Detection of soy in food from the Czech market using ELISA and PCR methods |
| title_short | Detection of soy in food from the Czech market using ELISA and PCR methods |
| title_sort | detection of soy in food from the czech market using elisa and pcr methods |
| topic | allergen processed product product labelling regulation no. 1169/2011 ctab method |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-202205-0006_detection-of-soy-in-food-from-the-czech-market-using-elisa-and-pcr-methods.php |
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