REV, a BRET-based sensor of ERK activity
Networks of signaling molecules are activated in response to environmental changes. How are these signaling networks dynamically integrated in space and time to process particular information? To tackle this issue, biosensors of single signaling pathways have been engineered. Bioluminescence resonan...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-07-01
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| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00095/full |
| _version_ | 1811310814258266112 |
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| author | Chanjuan eXu Marion ePeter nathalie ebouquier vincent eollendorff Ignacio eVillamil Jianfeng eLiu Laurent eFagni Julie ePerroy |
| author_facet | Chanjuan eXu Marion ePeter nathalie ebouquier vincent eollendorff Ignacio eVillamil Jianfeng eLiu Laurent eFagni Julie ePerroy |
| author_sort | Chanjuan eXu |
| collection | DOAJ |
| description | Networks of signaling molecules are activated in response to environmental changes. How are these signaling networks dynamically integrated in space and time to process particular information? To tackle this issue, biosensors of single signaling pathways have been engineered. Bioluminescence resonance energy transfer (BRET)-based biosensors have proven to be particularly efficient in that matter due to the high sensitivity of this technology to monitor protein-protein interactions or conformational changes in living cells. Extracellular signal-regulated kinases (ERK) are ubiquitously expressed and involved in many diverse cellular functions that might be encoded by the strength and spatio-temporal pattern of ERK activation. We developed a BRET-based sensor of ERK activity, called « REV » for Rluc8-ERKsubstrate-Venus. As expected, BRET changes of REV were correlated with ERK phosphorylation, which is required for its kinase activity. In neurons, the nature of the stimuli determines the strength, the location or the moment of ERK activation, thus highlighting how acute modulation of ERK may encode the nature of initial stimulus to specify the consequences of this activation. This study provides evidence for suitability of REV as a new biosensor to address biological questions. |
| first_indexed | 2024-04-13T10:06:35Z |
| format | Article |
| id | doaj.art-bf1a7eeb207a46888b7465edd585a24a |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T10:06:35Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-bf1a7eeb207a46888b7465edd585a24a2022-12-22T02:51:05ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922013-07-01410.3389/fendo.2013.0009555513REV, a BRET-based sensor of ERK activityChanjuan eXu0Marion ePeter1nathalie ebouquier2vincent eollendorff3Ignacio eVillamil4Jianfeng eLiu5Laurent eFagni6Julie ePerroy7Huazhong University of Science and TechnologyInstitut de Génétique Moléculaire de Montpellier (IGMM)Centre National de la Recherche ScientifiqueINRACentre National de la Recherche ScientifiqueHuazhong University of Science and TechnologyCentre National de la Recherche ScientifiqueCentre National de la Recherche ScientifiqueNetworks of signaling molecules are activated in response to environmental changes. How are these signaling networks dynamically integrated in space and time to process particular information? To tackle this issue, biosensors of single signaling pathways have been engineered. Bioluminescence resonance energy transfer (BRET)-based biosensors have proven to be particularly efficient in that matter due to the high sensitivity of this technology to monitor protein-protein interactions or conformational changes in living cells. Extracellular signal-regulated kinases (ERK) are ubiquitously expressed and involved in many diverse cellular functions that might be encoded by the strength and spatio-temporal pattern of ERK activation. We developed a BRET-based sensor of ERK activity, called « REV » for Rluc8-ERKsubstrate-Venus. As expected, BRET changes of REV were correlated with ERK phosphorylation, which is required for its kinase activity. In neurons, the nature of the stimuli determines the strength, the location or the moment of ERK activation, thus highlighting how acute modulation of ERK may encode the nature of initial stimulus to specify the consequences of this activation. This study provides evidence for suitability of REV as a new biosensor to address biological questions.http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00095/fullbiosensorBioluminescence resonance energy transfer (BRET)BRET imagingFluorescence-lifetime imaging microscopy (FLIM)Extracellular signal-regulated kinases (ERK)spatio-temporal signaling |
| spellingShingle | Chanjuan eXu Marion ePeter nathalie ebouquier vincent eollendorff Ignacio eVillamil Jianfeng eLiu Laurent eFagni Julie ePerroy REV, a BRET-based sensor of ERK activity Frontiers in Endocrinology biosensor Bioluminescence resonance energy transfer (BRET) BRET imaging Fluorescence-lifetime imaging microscopy (FLIM) Extracellular signal-regulated kinases (ERK) spatio-temporal signaling |
| title | REV, a BRET-based sensor of ERK activity |
| title_full | REV, a BRET-based sensor of ERK activity |
| title_fullStr | REV, a BRET-based sensor of ERK activity |
| title_full_unstemmed | REV, a BRET-based sensor of ERK activity |
| title_short | REV, a BRET-based sensor of ERK activity |
| title_sort | rev a bret based sensor of erk activity |
| topic | biosensor Bioluminescence resonance energy transfer (BRET) BRET imaging Fluorescence-lifetime imaging microscopy (FLIM) Extracellular signal-regulated kinases (ERK) spatio-temporal signaling |
| url | http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00095/full |
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