CRISPR-Cas12a: Functional overview and applications
Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Biomedical Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2319417019305050 |
| _version_ | 1811182747020951552 |
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| author | Bijoya Paul Guillermo Montoya |
| author_facet | Bijoya Paul Guillermo Montoya |
| author_sort | Bijoya Paul |
| collection | DOAJ |
| description | Prokaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution. Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape. In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in genome editing and biotechnology applications. Keywords: CRISPR-Cas12a, RNA guided endonucleases, crRNA biogenesis, Indiscriminate ssDNAse, Endonuclease recycling, Genome editing |
| first_indexed | 2024-04-11T09:36:02Z |
| format | Article |
| id | doaj.art-bf202925ac68460ba3468b3ca34f9be4 |
| institution | Directory Open Access Journal |
| issn | 2319-4170 |
| language | English |
| last_indexed | 2024-04-11T09:36:02Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Journal |
| spelling | doaj.art-bf202925ac68460ba3468b3ca34f9be42022-12-22T04:31:42ZengElsevierBiomedical Journal2319-41702020-02-01431817CRISPR-Cas12a: Functional overview and applicationsBijoya Paul0Guillermo Montoya1Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Copenhagen, DenmarkCorresponding author. Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B, Copenhagen 2200, Denmark.; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Copenhagen, DenmarkProkaryotes have developed an adaptive immune system called Clustered regularly interspaced short palindromic repeats (CRISPR) to combat attacks by foreign mobile genetic elements (MGEs) such as plasmids and phages. In the past decade, the widely characterized CRISPR-Cas9 enzyme has been redesigned to trigger a genome editing revolution. Class II type V CRISPR-Cas12a is a new RNA guided endonuclease that has been recently harnessed as an alternative genome editing tool, which is emerging as a powerful molecular scissor to consider in the genome editing application landscape. In this review, we aim to provide a mechanistic insight into the working mechanism of Cas12a, comparing it with Cas9, and eventually provide an overview of its current applications in genome editing and biotechnology applications. Keywords: CRISPR-Cas12a, RNA guided endonucleases, crRNA biogenesis, Indiscriminate ssDNAse, Endonuclease recycling, Genome editinghttp://www.sciencedirect.com/science/article/pii/S2319417019305050 |
| spellingShingle | Bijoya Paul Guillermo Montoya CRISPR-Cas12a: Functional overview and applications Biomedical Journal |
| title | CRISPR-Cas12a: Functional overview and applications |
| title_full | CRISPR-Cas12a: Functional overview and applications |
| title_fullStr | CRISPR-Cas12a: Functional overview and applications |
| title_full_unstemmed | CRISPR-Cas12a: Functional overview and applications |
| title_short | CRISPR-Cas12a: Functional overview and applications |
| title_sort | crispr cas12a functional overview and applications |
| url | http://www.sciencedirect.com/science/article/pii/S2319417019305050 |
| work_keys_str_mv | AT bijoyapaul crisprcas12afunctionaloverviewandapplications AT guillermomontoya crisprcas12afunctionaloverviewandapplications |