Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats
The present study is conducted to investigate the possible antidiabetic effect of certain phosphodiesterase inhibitors. Diabetes mellitus was induced in 18 h-fasted male wistar albino rats by intraperitoneal injection of streptozotocin (STZ) in a single dose (50 mg/kg). Gliclazide (Glcl) as a refere...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2014-12-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093114000337 |
| _version_ | 1828075767903289344 |
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| author | Mostafa El Sayed El Sayed Nehad Eid Ahmed Seif El Din Kamel |
| author_facet | Mostafa El Sayed El Sayed Nehad Eid Ahmed Seif El Din Kamel |
| author_sort | Mostafa El Sayed El Sayed |
| collection | DOAJ |
| description | The present study is conducted to investigate the possible antidiabetic effect of certain phosphodiesterase inhibitors. Diabetes mellitus was induced in 18 h-fasted male wistar albino rats by intraperitoneal injection of streptozotocin (STZ) in a single dose (50 mg/kg). Gliclazide (Glcl) as a reference standard in a dose of 10 mg/kg, sildenafil (Sild) in 3 doses (5, 10, 20 mg/kg) as PDE5 inhibitor and vinpocetine (Vinp) in 3 doses (10, 20, 40 mg/kg) as PDE1 inhibitor were injected intraperitoneal daily for 2 weeks. Their effects were assessed at different time intervals namely; 2 h after first dose, 1 week and 2 weeks after drug administration. In the present study, STZ significantly elevated serum blood glucose (SBG) level, lowered serum insulin, C-peptide levels and decreased liver glycogen content (LGC). Glcl significantly elevated serum insulin and C-peptide levels accompanied by reduction in serum glucose level and raised LGC. Vinp and Sild elevated serum insulin, C-peptide levels, LGC and decreased SBG level. The antidiabetic effect of Glcl was significantly higher than that of Vinp or Sild. It could be concluded that Vinp possibly produced its insulin stimulatory action via inhibition of PDE1 while Sild stimulated insulin secretion through inhibition of PDE5. The increase in serum C-peptide indicated that Vinp and Sild stimulated synthesis of insulin in the β-cells and Vinp is more potent than Sild in this respect due to the difference in their mechanism of action. |
| first_indexed | 2024-04-11T02:03:18Z |
| format | Article |
| id | doaj.art-bf31bbba2bfe4a74ab1ca9545d383c7a |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T02:03:18Z |
| publishDate | 2014-12-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-bf31bbba2bfe4a74ab1ca9545d383c7a2023-01-03T03:38:25ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312014-12-0152217918910.1016/j.bfopcu.2014.06.001Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in ratsMostafa El Sayed El SayedNehad EidAhmed Seif El Din KamelThe present study is conducted to investigate the possible antidiabetic effect of certain phosphodiesterase inhibitors. Diabetes mellitus was induced in 18 h-fasted male wistar albino rats by intraperitoneal injection of streptozotocin (STZ) in a single dose (50 mg/kg). Gliclazide (Glcl) as a reference standard in a dose of 10 mg/kg, sildenafil (Sild) in 3 doses (5, 10, 20 mg/kg) as PDE5 inhibitor and vinpocetine (Vinp) in 3 doses (10, 20, 40 mg/kg) as PDE1 inhibitor were injected intraperitoneal daily for 2 weeks. Their effects were assessed at different time intervals namely; 2 h after first dose, 1 week and 2 weeks after drug administration. In the present study, STZ significantly elevated serum blood glucose (SBG) level, lowered serum insulin, C-peptide levels and decreased liver glycogen content (LGC). Glcl significantly elevated serum insulin and C-peptide levels accompanied by reduction in serum glucose level and raised LGC. Vinp and Sild elevated serum insulin, C-peptide levels, LGC and decreased SBG level. The antidiabetic effect of Glcl was significantly higher than that of Vinp or Sild. It could be concluded that Vinp possibly produced its insulin stimulatory action via inhibition of PDE1 while Sild stimulated insulin secretion through inhibition of PDE5. The increase in serum C-peptide indicated that Vinp and Sild stimulated synthesis of insulin in the β-cells and Vinp is more potent than Sild in this respect due to the difference in their mechanism of action.http://www.sciencedirect.com/science/article/pii/S1110093114000337DiabetesStreptozotocinVinpocetineSildenafilInsulinC-peptide |
| spellingShingle | Mostafa El Sayed El Sayed Nehad Eid Ahmed Seif El Din Kamel Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats Bulletin of Faculty of Pharmacy Cairo University Diabetes Streptozotocin Vinpocetine Sildenafil Insulin C-peptide |
| title | Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats |
| title_full | Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats |
| title_fullStr | Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats |
| title_full_unstemmed | Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats |
| title_short | Beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats |
| title_sort | beneficial effects of certain phosphodiesterase inhibitors on diabetes mellitus in rats |
| topic | Diabetes Streptozotocin Vinpocetine Sildenafil Insulin C-peptide |
| url | http://www.sciencedirect.com/science/article/pii/S1110093114000337 |
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