Multiplexed mobilization and expression of biosynthetic gene clusters

Efficient mobilization and expression of biosynthetic gene clusters (BGCs) into heterologous hosts is needed for microbial natural products discovery. Here, the authors improve the CONKAT-seq strategy by simultaneously capturing the BCGs into a single large insert library and demonstrate its ability...

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Bibliographic Details
Main Authors: Vincent Libis, Logan W. MacIntyre, Rabia Mehmood, Liliana Guerrero, Melinda A. Ternei, Niv Antonovsky, Ján Burian, Zongqiang Wang, Sean F. Brady
Format: Article
Language:English
Published: Nature Portfolio 2022-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-32858-0