Multiplexed mobilization and expression of biosynthetic gene clusters
Efficient mobilization and expression of biosynthetic gene clusters (BGCs) into heterologous hosts is needed for microbial natural products discovery. Here, the authors improve the CONKAT-seq strategy by simultaneously capturing the BCGs into a single large insert library and demonstrate its ability...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-32858-0 |
| _version_ | 1811273557283438592 |
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| author | Vincent Libis Logan W. MacIntyre Rabia Mehmood Liliana Guerrero Melinda A. Ternei Niv Antonovsky Ján Burian Zongqiang Wang Sean F. Brady |
| author_facet | Vincent Libis Logan W. MacIntyre Rabia Mehmood Liliana Guerrero Melinda A. Ternei Niv Antonovsky Ján Burian Zongqiang Wang Sean F. Brady |
| author_sort | Vincent Libis |
| collection | DOAJ |
| description | Efficient mobilization and expression of biosynthetic gene clusters (BGCs) into heterologous hosts is needed for microbial natural products discovery. Here, the authors improve the CONKAT-seq strategy by simultaneously capturing the BCGs into a single large insert library and demonstrate its ability to discover natural products with new structures and potent antibacterial activity. |
| first_indexed | 2024-04-12T23:01:36Z |
| format | Article |
| id | doaj.art-bf50f0e3f20d48b3a29e45a4676ef6ac |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T23:01:36Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-bf50f0e3f20d48b3a29e45a4676ef6ac2022-12-22T03:13:03ZengNature PortfolioNature Communications2041-17232022-09-0113111010.1038/s41467-022-32858-0Multiplexed mobilization and expression of biosynthetic gene clustersVincent Libis0Logan W. MacIntyre1Rabia Mehmood2Liliana Guerrero3Melinda A. Ternei4Niv Antonovsky5Ján Burian6Zongqiang Wang7Sean F. Brady8Laboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityLaboratory of Genetically Encoded Small Molecules, The Rockefeller UniversityEfficient mobilization and expression of biosynthetic gene clusters (BGCs) into heterologous hosts is needed for microbial natural products discovery. Here, the authors improve the CONKAT-seq strategy by simultaneously capturing the BCGs into a single large insert library and demonstrate its ability to discover natural products with new structures and potent antibacterial activity.https://doi.org/10.1038/s41467-022-32858-0 |
| spellingShingle | Vincent Libis Logan W. MacIntyre Rabia Mehmood Liliana Guerrero Melinda A. Ternei Niv Antonovsky Ján Burian Zongqiang Wang Sean F. Brady Multiplexed mobilization and expression of biosynthetic gene clusters Nature Communications |
| title | Multiplexed mobilization and expression of biosynthetic gene clusters |
| title_full | Multiplexed mobilization and expression of biosynthetic gene clusters |
| title_fullStr | Multiplexed mobilization and expression of biosynthetic gene clusters |
| title_full_unstemmed | Multiplexed mobilization and expression of biosynthetic gene clusters |
| title_short | Multiplexed mobilization and expression of biosynthetic gene clusters |
| title_sort | multiplexed mobilization and expression of biosynthetic gene clusters |
| url | https://doi.org/10.1038/s41467-022-32858-0 |
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