Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators

Bi-FoRe: an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators

Abstract Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene function. However, contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been re...

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Bibliographic Details
Main Authors: Bingzhou Han, Yage Zhang, Xuetong Bi, Yang Zhou, Christopher J. Krueger, Xinli Hu, Zuoyan Zhu, Xiangjun Tong, Bo Zhang
Format: Article
Language:English
Published: Oxford University Press 2020-07-01
Series:Protein & Cell
Subjects:
CRISPR/Cas
conditional knockout
allele labeling
conditional rescue
minicircle DNA
Online Access:https://doi.org/10.1007/s13238-020-00747-1

Internet

https://doi.org/10.1007/s13238-020-00747-1

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