Efficiency Optimization of CRISPR/Cas9-Mediated Targeted Mutagenesis in Grape

Efficiency Optimization of CRISPR/Cas9-Mediated Targeted Mutagenesis in Grape

Clustered regularly interspersed short palindromic repeats (CRISPR)/Cas system is an efficient targeted genome editing method. Although CRISPR/Cas9-mediated mutagenesis has been applied successfully in grape, few studies have examined the technique’s efficiency. To optimize CRISPR/Cas9 editing effic...

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Bibliographic Details
Main Authors: Fengrui Ren, Chong Ren, Zhan Zhang, Wei Duan, David Lecourieux, Shaohua Li, Zhenchang Liang
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-05-01
Series:Frontiers in Plant Science
Subjects:
CRISPR/Cas9
optimization
GC content
grape
gene expression
gene editing efficiency
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2019.00612/full

Internet

https://www.frontiersin.org/article/10.3389/fpls.2019.00612/full

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