Optimization of antigen-specific CD8+ T cell activation conditions for infectious diseases including COVID-19

Optimization of antigen-specific CD8+ T cell activation conditions for infectious diseases including COVID-19

Summary: Here, we describe the use of the artificial antigen-presenting cell (aAPC) system for the verification of T-cell epitopes. We purify and activate CD8+ T cells from blood samples from HLA-A2 that are negative for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). CD8+ T cells are...

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Bibliographic Details
Main Authors: Chanchan Xiao, Congling Qiu, Jieping Deng, Jiezhou Ye, Lijuan Gao, Jun Su, Oscar Junhong Luo, Pengcheng Wang, Guobing Chen
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Immunology
Microbiology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004950

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http://www.sciencedirect.com/science/article/pii/S2666166721004950

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