In vitro flow cytometry assay to assess primary human and mouse macrophage phagocytosis of live cells

Summary: Although tumor-associated macrophages are generally immunosuppressive, macrophages may also promote tumor clearance via phagocytosis of live tumor cells. Here, we present a protocol for assessing macrophage engulfment of tumor cells in vitro using flow cytometry. We describe steps for cell...

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Bibliographic Details
Main Authors: Samantha Y. Liu, Naomi Mulugeta, Stephanie K. Dougan, Li Qiang
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001983