In vitro flow cytometry assay to assess primary human and mouse macrophage phagocytosis of live cells
Summary: Although tumor-associated macrophages are generally immunosuppressive, macrophages may also promote tumor clearance via phagocytosis of live tumor cells. Here, we present a protocol for assessing macrophage engulfment of tumor cells in vitro using flow cytometry. We describe steps for cell...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2023-06-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723001983 |