High-Survival Rate After Microinjection of Mouse Oocytes and Early Embryos With mRNA by Combining a Tip Pipette and Piezoelectric-Assisted Micromanipulator

High-Survival Rate After Microinjection of Mouse Oocytes and Early Embryos With mRNA by Combining a Tip Pipette and Piezoelectric-Assisted Micromanipulator

Utilizing microinjection to introduce biological molecules such as DNA, mRNA, siRNA, and proteins into the cell is well established to study oocyte maturation and early embryo development in vitro. However, microinjection is an empirical technology. The cellular survival after microinjection is main...

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Bibliographic Details
Main Authors: Lei-Ning Chen, Xiao-Yan Fan, Yi-Tong Liu, Shao-Qing Chen, Feng-Yun Xie, Li Zeng, Juan Wen, Jin Li, Jun-Yu Ma, Xiang-Hong Ou, Shi-Ming Luo, Lei Guo
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
microinjection
cumulus–oocyte complex
GV oocyte
MII oocyte
zygote
two-cell embryo
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2021.735971/full

Internet

https://www.frontiersin.org/articles/10.3389/fcell.2021.735971/full

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