Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis

Evaluation of full-length nanopore 16S sequencing for detection of pathogens in microbial keratitis

Background Microbial keratitis is a leading cause of preventable blindness worldwide. Conventional sampling and culture techniques are time-consuming, with over 40% of cases being culture-negative. Nanopore sequencing technology is portable and capable of generating long sequencing reads in real-tim...

Full description

Bibliographic Details
Main Authors: Liying Low, Pablo Fuentes-Utrilla, James Hodson, John D. O’Neil, Amanda E. Rossiter, Ghazala Begum, Kusy Suleiman, Philip I. Murray, Graham R. Wallace, Nicholas J. Loman, Saaeha Rauz, West Midlands Collaborative Ophthalmology Network for Clinical Effectiveness & Research by Trainees (WM CONCERT)
Format: Article
Language:English
Published: PeerJ Inc. 2021-02-01
Series:PeerJ
Subjects:
Nanopore sequencing
Eye infection
Microbial keratitis
Full length 16S rRNA sequencing
Cornea infection
Eye swab
Online Access:https://peerj.com/articles/10778.pdf

Internet

https://peerj.com/articles/10778.pdf

Similar Items