Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα
Abstract Background Hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, which includes bone-forming and bone-resorbing cells, i.e., osteoblasts (OBs) and osteoclasts (OCs). OBs originate from mesenchymal progenitors, while OCs are derived from HSCs. Self-renewal, proliferation and...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | Cell & Bioscience |
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| Online Access: | https://doi.org/10.1186/s13578-023-01067-7 |
| _version_ | 1797795473591894016 |
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| author | Alessia Oppezzo Lovely Monney Henri Kilian Lofti Slimani Frédérique Maczkowiak-Chartois Filippo Rosselli |
| author_facet | Alessia Oppezzo Lovely Monney Henri Kilian Lofti Slimani Frédérique Maczkowiak-Chartois Filippo Rosselli |
| author_sort | Alessia Oppezzo |
| collection | DOAJ |
| description | Abstract Background Hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, which includes bone-forming and bone-resorbing cells, i.e., osteoblasts (OBs) and osteoclasts (OCs). OBs originate from mesenchymal progenitors, while OCs are derived from HSCs. Self-renewal, proliferation and differentiation of HSCs are under the control of regulatory signals generated by OBs and OCs within the BM niche. Consequently, OBs and OCs control both bone physiology and hematopoiesis. Since the human developmental and bone marrow failure genetic syndrome fanconi anemia (FA) presents with skeletal abnormalities, osteoporosis and HSC impairment, we wanted to test the hypothesis that the main pathological abnormalities of FA could be related to a defect in OC physiology and/or in bone homeostasis. Results We revealed here that the intrinsic differentiation of OCs from a Fanca −/− mouse is impaired in vitro due to overactivation of the p53–p21 axis and defects in NF-kB signaling. The OC differentiation abnormalities observed in vitro were rescued by treating Fanca −/− cells with the p53 inhibitor pifithrin-α, by treatment with the proinflammatory cytokine TNFα or by coculturing them with Fanca-proficient or Fanca-deficient osteoblastic cells. Conclusions Overall, our results highlight an unappreciated role of Fanca in OC differentiation that is potentially circumvented in vivo by the presence of OBs and TNFα in the BM niche. |
| first_indexed | 2024-03-13T03:18:30Z |
| format | Article |
| id | doaj.art-bfd377a506b04c929a5c17294a790623 |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-03-13T03:18:30Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-bfd377a506b04c929a5c17294a7906232023-06-25T11:30:26ZengBMCCell & Bioscience2045-37012023-06-0113111710.1186/s13578-023-01067-7Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFαAlessia Oppezzo0Lovely Monney1Henri Kilian2Lofti Slimani3Frédérique Maczkowiak-Chartois4Filippo Rosselli5CNRS UMR9019, Équipe labellisée La Ligue contre le CancerCNRS UMR9019, Équipe labellisée La Ligue contre le CancerURP2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant (PIV), FHU-DDS-net, Dental School, Université de ParisURP2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant (PIV), FHU-DDS-net, Dental School, Université de ParisCNRS UMR9019, Équipe labellisée La Ligue contre le CancerCNRS UMR9019, Équipe labellisée La Ligue contre le CancerAbstract Background Hematopoietic stem cells (HSCs) reside in the bone marrow (BM) niche, which includes bone-forming and bone-resorbing cells, i.e., osteoblasts (OBs) and osteoclasts (OCs). OBs originate from mesenchymal progenitors, while OCs are derived from HSCs. Self-renewal, proliferation and differentiation of HSCs are under the control of regulatory signals generated by OBs and OCs within the BM niche. Consequently, OBs and OCs control both bone physiology and hematopoiesis. Since the human developmental and bone marrow failure genetic syndrome fanconi anemia (FA) presents with skeletal abnormalities, osteoporosis and HSC impairment, we wanted to test the hypothesis that the main pathological abnormalities of FA could be related to a defect in OC physiology and/or in bone homeostasis. Results We revealed here that the intrinsic differentiation of OCs from a Fanca −/− mouse is impaired in vitro due to overactivation of the p53–p21 axis and defects in NF-kB signaling. The OC differentiation abnormalities observed in vitro were rescued by treating Fanca −/− cells with the p53 inhibitor pifithrin-α, by treatment with the proinflammatory cytokine TNFα or by coculturing them with Fanca-proficient or Fanca-deficient osteoblastic cells. Conclusions Overall, our results highlight an unappreciated role of Fanca in OC differentiation that is potentially circumvented in vivo by the presence of OBs and TNFα in the BM niche.https://doi.org/10.1186/s13578-023-01067-7Fanconi anemiaOsteoclastOsteoblastCell signalingTNFαp53 |
| spellingShingle | Alessia Oppezzo Lovely Monney Henri Kilian Lofti Slimani Frédérique Maczkowiak-Chartois Filippo Rosselli Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα Cell & Bioscience Fanconi anemia Osteoclast Osteoblast Cell signaling TNFα p53 |
| title | Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα |
| title_full | Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα |
| title_fullStr | Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα |
| title_full_unstemmed | Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα |
| title_short | Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNFα |
| title_sort | fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by tnfα |
| topic | Fanconi anemia Osteoclast Osteoblast Cell signaling TNFα p53 |
| url | https://doi.org/10.1186/s13578-023-01067-7 |
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