Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections
Summary: Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations is challenging. To overcome this, we rationally select the highly conserved peptides from the consens...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723007775 |
| _version_ | 1797784922772996096 |
|---|---|
| author | De-Jian Liu Cui-Cui Liu Xiu-Qin Zhong Xuan Wu Hui-Hui Zhang Shang-Wen Lu Zhuo-Ling Shen Wen-Wen Song Shi-Long Zhao You-Song Peng He-Ping Zheng Mu-Yang Wan Yao-Qing Chen Lei Deng |
| author_facet | De-Jian Liu Cui-Cui Liu Xiu-Qin Zhong Xuan Wu Hui-Hui Zhang Shang-Wen Lu Zhuo-Ling Shen Wen-Wen Song Shi-Long Zhao You-Song Peng He-Ping Zheng Mu-Yang Wan Yao-Qing Chen Lei Deng |
| author_sort | De-Jian Liu |
| collection | DOAJ |
| description | Summary: Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations is challenging. To overcome this, we rationally select the highly conserved peptides from the consensus amino acid sequence of the globular head domains of neuraminidase. Inspired by the B cell receptor evolution process, a reliable sequential immunization regimen is designed to result in immuno-focusing by steering bulk immune responses to a selected region where broadly protective B lymphocyte epitopes reside. After priming neuraminidase protein-specific antibody responses in C57BL/6 or BALB/c inbred mice strains by immunization or pre-infection, boost immunizations with certain neuraminidase-derived peptide-keyhole limpet hemocyanin conjugates significantly strengthened serum neuraminidase inhibition activities and cross-protections. Overall, this study provides proof of concept for a peptide-based sequential immunization strategy for achieving targeted induction of cross-protective antibody response, which provides references for designing universal vaccines against other highly variable pathogens. |
| first_indexed | 2024-03-13T00:46:46Z |
| format | Article |
| id | doaj.art-bffb56e15a104568b1a4454a559d1019 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-13T00:46:46Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-bffb56e15a104568b1a4454a559d10192023-07-09T04:21:44ZengElsevierCell Reports2211-12472023-07-01427112766Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protectionsDe-Jian Liu0Cui-Cui Liu1Xiu-Qin Zhong2Xuan Wu3Hui-Hui Zhang4Shang-Wen Lu5Zhuo-Ling Shen6Wen-Wen Song7Shi-Long Zhao8You-Song Peng9He-Ping Zheng10Mu-Yang Wan11Yao-Qing Chen12Lei Deng13Hunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaBioinformatics Center, College of Biology, Hunan University, Changsha 410082, ChinaBioinformatics Center, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China; Bioinformatics Center, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China; Bioinformatics Center, College of Biology, Hunan University, Changsha 410082, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, ChinaSchool of Public Health (Shenzhen), Sun Yat-Sen University, Shenzhen, Guangdong Province 518107, ChinaHunan Provincial Key Laboratory of Medical Virology, College of Biology, Hunan University, Changsha 410082, China; Bioinformatics Center, College of Biology, Hunan University, Changsha 410082, China; Beijing Weimiao Biotechnology Co., Ltd., Haidian District, Beijing 100000, China; Corresponding authorSummary: Neuraminidase is suggested as an important component for developing a universal influenza vaccine. Targeted induction of neuraminidase-specific broadly protective antibodies by vaccinations is challenging. To overcome this, we rationally select the highly conserved peptides from the consensus amino acid sequence of the globular head domains of neuraminidase. Inspired by the B cell receptor evolution process, a reliable sequential immunization regimen is designed to result in immuno-focusing by steering bulk immune responses to a selected region where broadly protective B lymphocyte epitopes reside. After priming neuraminidase protein-specific antibody responses in C57BL/6 or BALB/c inbred mice strains by immunization or pre-infection, boost immunizations with certain neuraminidase-derived peptide-keyhole limpet hemocyanin conjugates significantly strengthened serum neuraminidase inhibition activities and cross-protections. Overall, this study provides proof of concept for a peptide-based sequential immunization strategy for achieving targeted induction of cross-protective antibody response, which provides references for designing universal vaccines against other highly variable pathogens.http://www.sciencedirect.com/science/article/pii/S2211124723007775CP: Immunology |
| spellingShingle | De-Jian Liu Cui-Cui Liu Xiu-Qin Zhong Xuan Wu Hui-Hui Zhang Shang-Wen Lu Zhuo-Ling Shen Wen-Wen Song Shi-Long Zhao You-Song Peng He-Ping Zheng Mu-Yang Wan Yao-Qing Chen Lei Deng Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections Cell Reports CP: Immunology |
| title | Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections |
| title_full | Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections |
| title_fullStr | Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections |
| title_full_unstemmed | Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections |
| title_short | Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections |
| title_sort | boost immunizations with na derived peptide conjugates achieve induction of na inhibition antibodies and heterologous influenza protections |
| topic | CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723007775 |
| work_keys_str_mv | AT dejianliu boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT cuicuiliu boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT xiuqinzhong boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT xuanwu boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT huihuizhang boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT shangwenlu boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT zhuolingshen boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT wenwensong boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT shilongzhao boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT yousongpeng boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT hepingzheng boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT muyangwan boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT yaoqingchen boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections AT leideng boostimmunizationswithnaderivedpeptideconjugatesachieveinductionofnainhibitionantibodiesandheterologousinfluenzaprotections |