DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells
Quaternary derivatives of 1,4-diazabicyclo[2.2.2]octane (DABCO) and of quinuclidine surfactants were used to develop oil-in-water nanoemulsions with the purpose of selecting the best long-term stable nanoemulsion for the ocular administration of triamcinolone acetonide (TA). The combination of the b...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/10/1652 |
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| author | Ana R. Fernandes Tiago dos Santos Pedro L. Granja Elena Sanchez-Lopez Antonello Santini Maria L. Garcia Amelia M. Silva Eliana B. Souto |
| author_facet | Ana R. Fernandes Tiago dos Santos Pedro L. Granja Elena Sanchez-Lopez Antonello Santini Maria L. Garcia Amelia M. Silva Eliana B. Souto |
| author_sort | Ana R. Fernandes |
| collection | DOAJ |
| description | Quaternary derivatives of 1,4-diazabicyclo[2.2.2]octane (DABCO) and of quinuclidine surfactants were used to develop oil-in-water nanoemulsions with the purpose of selecting the best long-term stable nanoemulsion for the ocular administration of triamcinolone acetonide (TA). The combination of the best physicochemical properties (i.e., mean droplet size, polydispersity index, zeta potential, osmolality, viscoelastic properties, surface tension) was considered, together with the cell viability assays in ARPE-19 and HMC3 cell lines. Surfactants with cationic properties have been used to tailor the nanoemulsions’ surface for site-specific delivery of drugs to the ocular structure for the delivery of TA. They are tailored for the eye because they have cationic properties that interact with the anionic surface of the eye. |
| first_indexed | 2024-03-10T06:16:34Z |
| format | Article |
| id | doaj.art-bffef06e78ba4fa6b311c0fffb972e17 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T06:16:34Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-bffef06e78ba4fa6b311c0fffb972e172023-11-22T19:39:11ZengMDPI AGPharmaceutics1999-49232021-10-011310165210.3390/pharmaceutics13101652DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia CellsAna R. Fernandes0Tiago dos Santos1Pedro L. Granja2Elena Sanchez-Lopez3Antonello Santini4Maria L. Garcia5Amelia M. Silva6Eliana B. Souto7i3s—Institute for Research & Innovation in Health, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugali3s—Institute for Research & Innovation in Health, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugali3s—Institute for Research & Innovation in Health, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, PortugalDepartment of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, SpainDepartment of Pharmacy, Università di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, ItalyDepartment of Pharmacy, Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, 08028 Barcelona, SpainCentre for Research and Technology of Agro-Environmental and Biological Sciences, CITAB, UTAD, Quinta de Prados, 5001-801 Vila Real, PortugalCEB—Centre of Biological Engineering, Campus de Gualtar, University of Minho, 4710-057 Braga, PortugalQuaternary derivatives of 1,4-diazabicyclo[2.2.2]octane (DABCO) and of quinuclidine surfactants were used to develop oil-in-water nanoemulsions with the purpose of selecting the best long-term stable nanoemulsion for the ocular administration of triamcinolone acetonide (TA). The combination of the best physicochemical properties (i.e., mean droplet size, polydispersity index, zeta potential, osmolality, viscoelastic properties, surface tension) was considered, together with the cell viability assays in ARPE-19 and HMC3 cell lines. Surfactants with cationic properties have been used to tailor the nanoemulsions’ surface for site-specific delivery of drugs to the ocular structure for the delivery of TA. They are tailored for the eye because they have cationic properties that interact with the anionic surface of the eye.https://www.mdpi.com/1999-4923/13/10/1652cationic nanoemulsionsDABCO surfactantsquinuclidine surfactantsocular administrationtriamcinolone acetonideage-related macular degeneration |
| spellingShingle | Ana R. Fernandes Tiago dos Santos Pedro L. Granja Elena Sanchez-Lopez Antonello Santini Maria L. Garcia Amelia M. Silva Eliana B. Souto DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells Pharmaceutics cationic nanoemulsions DABCO surfactants quinuclidine surfactants ocular administration triamcinolone acetonide age-related macular degeneration |
| title | DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells |
| title_full | DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells |
| title_fullStr | DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells |
| title_full_unstemmed | DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells |
| title_short | DABCO-Customized Nanoemulsions: Characterization, Cell Viability and Genotoxicity in Retinal Pigmented Epithelium and Microglia Cells |
| title_sort | dabco customized nanoemulsions characterization cell viability and genotoxicity in retinal pigmented epithelium and microglia cells |
| topic | cationic nanoemulsions DABCO surfactants quinuclidine surfactants ocular administration triamcinolone acetonide age-related macular degeneration |
| url | https://www.mdpi.com/1999-4923/13/10/1652 |
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