Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice

Abstract The classical organ culture method, in which tissue is placed at the gas‒liquid interphase, is effective at inducing mouse spermatogenesis. However, due to reginal variations in the supply of oxygen and nutrients within a tissue, the progress of spermatogenesis was observed only in limited...

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Bibliographic Details
Main Authors: Kiyoshi Hashimoto, Hisakazu Odaka, Yu Ishikawa-Yamauchi, Shino Nagata, Hiroko Nakamura, Hiroshi Kimura, Takuya Sato, Kazuhide Makiyama, Takehiko Ogawa
Format: Article
Language:English
Published: Nature Portfolio 2023-07-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-39323-y