Protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z and H4K20Ecx for structure determination

Summary: The histone lysine methyltransferase SUV420H1 preferentially targets the H2A.Z-containing nucleosome core particle (H2A.Z-NCP) and catalyzes the H4K20me2 modification at replication origins. Here, we present a protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z a...

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Bibliografske podrobnosti
Main Authors: Li Huang, Zheng Zhou
Format: Article
Jezik:English
Izdano: Elsevier 2024-09-01
Serija:STAR Protocols
Teme:
Online dostop:http://www.sciencedirect.com/science/article/pii/S266616672400460X