Protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z and H4K20Ecx for structure determination

Summary: The histone lysine methyltransferase SUV420H1 preferentially targets the H2A.Z-containing nucleosome core particle (H2A.Z-NCP) and catalyzes the H4K20me2 modification at replication origins. Here, we present a protocol for preparing SUV420H1 in complex with the nucleosome containing H2A.Z a...

Celý popis

Podrobná bibliografie
Hlavní autoři: Li Huang, Zheng Zhou
Médium: Článek
Jazyk:English
Vydáno: Elsevier 2024-09-01
Edice:STAR Protocols
Témata:
On-line přístup:http://www.sciencedirect.com/science/article/pii/S266616672400460X