Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats
The phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways mediate β cell growth, proliferation, survival and death. We investigated whether protein restriction during pregnancy alters islet morphometry or the expression and phosphorylation of several proteins involved in the ph...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2015-06-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201007&lng=en&tlng=en |
| _version_ | 1818216777313681408 |
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| author | CRISTIANA S.B. SALVATIERRA SÍLVIA R.L. REIS ANA F.M. PESSOA LETÍCIA M.I. DE SOUZA LUIZ F. STOPPIGLIA ROBERTO V. VELOSO MARISE A.B. REIS EVERARDO M. CARNEIRO ANTONIO C. BOSCHERO EDSON M. COLODEL VANESSA C. ARANTES MÁRCIA Q. LATORRACA |
| author_facet | CRISTIANA S.B. SALVATIERRA SÍLVIA R.L. REIS ANA F.M. PESSOA LETÍCIA M.I. DE SOUZA LUIZ F. STOPPIGLIA ROBERTO V. VELOSO MARISE A.B. REIS EVERARDO M. CARNEIRO ANTONIO C. BOSCHERO EDSON M. COLODEL VANESSA C. ARANTES MÁRCIA Q. LATORRACA |
| author_sort | CRISTIANA S.B. SALVATIERRA |
| collection | DOAJ |
| description | The phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways mediate β cell growth, proliferation, survival and death. We investigated whether protein restriction during pregnancy alters islet morphometry or the expression and phosphorylation of several proteins involved in the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways. As controls, adult pregnant and non-pregnant rats were fed a normal-protein diet (17%). Pregnant and non-pregnant rats in the experimental groups were fed a low-protein diet (6%) for 15 days. Low protein diet during pregnancy increased serum prolactin level, reduced serum corticosterone concentration and the expression of both protein kinase B/AKT1 (AKT1) and p70 ribosomal protein S6 kinase (p70S6K), as well as the islets area, but did not alter the insulin content of pancreatic islets. Pregnancy increased the expression of the Src homology/collagen (SHC) protein and the extracellular signal-regulated kinases 1/2 (ERK1/2) independent of diet. ERK1/2 phosphorylation (pERK1/2) was similar in islets from pregnant and non-pregnant rats fed a low-protein diet, and was higher in islets from pregnant rats than in islets from non-pregnant rats fed a normal-protein diet. Thus, a short-term, low-protein diet during pregnancy was sufficient to reduce the levels of proteins in the phosphatidylinositol 3-kinase pathway and affect islet morphometry. |
| first_indexed | 2024-12-12T06:57:22Z |
| format | Article |
| id | doaj.art-c0470ea9e5b540ebb90022d7cbaeb145 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-12T06:57:22Z |
| publishDate | 2015-06-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-c0470ea9e5b540ebb90022d7cbaeb1452022-12-22T00:33:56ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902015-06-018721007101810.1590/0001-3765201520140251S0001-37652015000201007Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in ratsCRISTIANA S.B. SALVATIERRASÍLVIA R.L. REISANA F.M. PESSOALETÍCIA M.I. DE SOUZALUIZ F. STOPPIGLIAROBERTO V. VELOSOMARISE A.B. REISEVERARDO M. CARNEIROANTONIO C. BOSCHEROEDSON M. COLODELVANESSA C. ARANTESMÁRCIA Q. LATORRACAThe phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways mediate β cell growth, proliferation, survival and death. We investigated whether protein restriction during pregnancy alters islet morphometry or the expression and phosphorylation of several proteins involved in the phosphatidylinositol 3-kinase and mitogen-activated protein kinase pathways. As controls, adult pregnant and non-pregnant rats were fed a normal-protein diet (17%). Pregnant and non-pregnant rats in the experimental groups were fed a low-protein diet (6%) for 15 days. Low protein diet during pregnancy increased serum prolactin level, reduced serum corticosterone concentration and the expression of both protein kinase B/AKT1 (AKT1) and p70 ribosomal protein S6 kinase (p70S6K), as well as the islets area, but did not alter the insulin content of pancreatic islets. Pregnancy increased the expression of the Src homology/collagen (SHC) protein and the extracellular signal-regulated kinases 1/2 (ERK1/2) independent of diet. ERK1/2 phosphorylation (pERK1/2) was similar in islets from pregnant and non-pregnant rats fed a low-protein diet, and was higher in islets from pregnant rats than in islets from non-pregnant rats fed a normal-protein diet. Thus, a short-term, low-protein diet during pregnancy was sufficient to reduce the levels of proteins in the phosphatidylinositol 3-kinase pathway and affect islet morphometry.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201007&lng=en&tlng=envia da MAP-cinaseilhotas pancreáticasvia da PI3-cinaseprenhezrestrição de proteínaratos |
| spellingShingle | CRISTIANA S.B. SALVATIERRA SÍLVIA R.L. REIS ANA F.M. PESSOA LETÍCIA M.I. DE SOUZA LUIZ F. STOPPIGLIA ROBERTO V. VELOSO MARISE A.B. REIS EVERARDO M. CARNEIRO ANTONIO C. BOSCHERO EDSON M. COLODEL VANESSA C. ARANTES MÁRCIA Q. LATORRACA Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats Anais da Academia Brasileira de Ciências via da MAP-cinase ilhotas pancreáticas via da PI3-cinase prenhez restrição de proteína ratos |
| title | Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats |
| title_full | Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats |
| title_fullStr | Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats |
| title_full_unstemmed | Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats |
| title_short | Short-term low-protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3-kinase pathway in rats |
| title_sort | short term low protein diet during pregnancy alters islet area and protein content of phosphatidylinositol 3 kinase pathway in rats |
| topic | via da MAP-cinase ilhotas pancreáticas via da PI3-cinase prenhez restrição de proteína ratos |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000201007&lng=en&tlng=en |
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