Purification, Identification and Polyclonal Antibody Development for Cow’s Milk Major Allergen αS1-Casein

Purification, Identification and Polyclonal Antibody Development for Cow’s Milk Major Allergen αS1-Casein

Objective: To purify and comprehensive identify αS1-casein from bovine caseins and prepare rabbit polyclonal antibody against αS1-casein. Method: DEAE Sepharose Fast Flow anion exchange chromatography was used to separate and purify αS1-casein. The physicochemical property (isoelectric point and pro...

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Bibliographic Details
Main Authors: Shengfa HE, Caiyun LONG, Jiao WANG, Meng XIONG, Jiangqiang ZHAO, Yan YAN, Yaping DONG, Xin LI, Hongbing CHEN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2022-08-01
Series:Shipin gongye ke-ji
Subjects:
cow’s milk allergen
αs1-casein
purification
identification
polyclonal antibody
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110034

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http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021110034

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