Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans
I report a method for single nucleotide polymorphism (SNP) detection and linkage mapping in Caenorhabditis elegans using automated oligonucleotide design and fluorescence-based quantitative PCR detection. Nine hundred twenty-three oligonucleotide pairs were designed to produce small products of <...
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2006-11-01
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Series: | BioTechniques |
Online Access: | https://www.future-science.com/doi/10.2144/000112256 |