Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans

I report a method for single nucleotide polymorphism (SNP) detection and linkage mapping in Caenorhabditis elegans using automated oligonucleotide design and fluorescence-based quantitative PCR detection. Nine hundred twenty-three oligonucleotide pairs were designed to produce small products of <...

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Bibliographic Details
Main Author: Christopher A. Shelton
Format: Article
Language:English
Published: Taylor & Francis Group 2006-11-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112256