Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials

Development of a novel directly marketable beer brewed at low temperature in a domestic refrigerator combined with yeast immobilization technology is presented in this study. Separately, freeze-dried wort and immobilized cells of the cryotolerant yeast strain Saccharomyces cerevisiae AXAZ-1 on tubul...

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Main Authors: Angelika-Ioanna Gialleli, Vassilios Ganatsios, Antonia Terpou, Maria Kanellaki, Argyro Bekatorou, Athanasios A. Koutinas, Dimitra Dimitrellou
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2017-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:https://hrcak.srce.hr/file/275137
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author Angelika-Ioanna Gialleli
Vassilios Ganatsios
Antonia Terpou
Maria Kanellaki
Argyro Bekatorou
Athanasios A. Koutinas
Dimitra Dimitrellou
author_facet Angelika-Ioanna Gialleli
Vassilios Ganatsios
Antonia Terpou
Maria Kanellaki
Argyro Bekatorou
Athanasios A. Koutinas
Dimitra Dimitrellou
author_sort Angelika-Ioanna Gialleli
collection DOAJ
description Development of a novel directly marketable beer brewed at low temperature in a domestic refrigerator combined with yeast immobilization technology is presented in this study. Separately, freeze-dried wort and immobilized cells of the cryotolerant yeast strain Saccharomyces cerevisiae AXAZ-1 on tubular cellulose were used in low-temperature fermentation (2, 5 and 7 °C). The positive effect of tubular cellulose during low-temperature brewing was examined, revealing that freeze-dried immobilized yeast cells on tubular cellulose significantly reduced the fermentation rates in contrast to freeze-dried free cells, although they are recommended for home-made beer production. Immobilization also enhanced the yeast resistance at low-temperature fermentation, reducing the minimum brewing temperature value from 5 to 2 °C. In the case of high-quality beer production, the effect of temperature and initial sugar concentration on the fermentation kinetics were assessed. Sensory enrichment of the produced beer was confirmed by the analysis of the final products, revealing a low diacetyl concentration, together with improved polyphenol content, aroma profile and clarity. The proposed process for beer production in a domestic refrigerator can easily be commercialized and applied by dissolving the content of two separate packages in tap water; one package containing dried wort and the other dried immobilized cells on tubular cellulose suspended in tap water.
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spelling doaj.art-c083baa313c94e7988e5f83f0819d6f22024-04-15T14:23:11ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062017-01-0155332533210.17113/ftb.55.03.17.4907Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw MaterialsAngelika-Ioanna Gialleli0Vassilios Ganatsios1Antonia Terpou2Maria Kanellaki3Argyro Bekatorou4Athanasios A. Koutinas5Dimitra Dimitrellou6Department of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDepartment of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDepartment of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDepartment of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDepartment of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDepartment of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDepartment of Chemistry, Food Biotechnology Group, University of Patras, GR-26500 Patras, GreeceDevelopment of a novel directly marketable beer brewed at low temperature in a domestic refrigerator combined with yeast immobilization technology is presented in this study. Separately, freeze-dried wort and immobilized cells of the cryotolerant yeast strain Saccharomyces cerevisiae AXAZ-1 on tubular cellulose were used in low-temperature fermentation (2, 5 and 7 °C). The positive effect of tubular cellulose during low-temperature brewing was examined, revealing that freeze-dried immobilized yeast cells on tubular cellulose significantly reduced the fermentation rates in contrast to freeze-dried free cells, although they are recommended for home-made beer production. Immobilization also enhanced the yeast resistance at low-temperature fermentation, reducing the minimum brewing temperature value from 5 to 2 °C. In the case of high-quality beer production, the effect of temperature and initial sugar concentration on the fermentation kinetics were assessed. Sensory enrichment of the produced beer was confirmed by the analysis of the final products, revealing a low diacetyl concentration, together with improved polyphenol content, aroma profile and clarity. The proposed process for beer production in a domestic refrigerator can easily be commercialized and applied by dissolving the content of two separate packages in tap water; one package containing dried wort and the other dried immobilized cells on tubular cellulose suspended in tap water.https://hrcak.srce.hr/file/275137brewinglow-temperature fermentationnovel technologyimmobilized cellsfreeze-dryingtubular cellulose
spellingShingle Angelika-Ioanna Gialleli
Vassilios Ganatsios
Antonia Terpou
Maria Kanellaki
Argyro Bekatorou
Athanasios A. Koutinas
Dimitra Dimitrellou
Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials
Food Technology and Biotechnology
brewing
low-temperature fermentation
novel technology
immobilized cells
freeze-drying
tubular cellulose
title Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials
title_full Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials
title_fullStr Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials
title_full_unstemmed Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials
title_short Technological Development of Brewing in Domestic Refrigerator Using Freeze-Dried Raw Materials
title_sort technological development of brewing in domestic refrigerator using freeze dried raw materials
topic brewing
low-temperature fermentation
novel technology
immobilized cells
freeze-drying
tubular cellulose
url https://hrcak.srce.hr/file/275137
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AT mariakanellaki technologicaldevelopmentofbrewingindomesticrefrigeratorusingfreezedriedrawmaterials
AT argyrobekatorou technologicaldevelopmentofbrewingindomesticrefrigeratorusingfreezedriedrawmaterials
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