Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and Its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids

Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and Its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids

Pairs of pyrrolysyl-tRNA synthetase (PylRS) and tRNA<sup>Pyl</sup> from <i>Methanosarcina mazei</i> and <i>Methanosarcina barkeri</i> are widely used for site-specific incorporations of non-canonical amino acids into proteins (genetic code expansion). Previously,...

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Bibliographic Details
Main Authors: Tatsuo Yanagisawa, Eiko Seki, Hiroaki Tanabe, Yoshifumi Fujii, Kensaku Sakamoto, Shigeyuki Yokoyama
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:International Journal of Molecular Sciences
Subjects:
non-canonical amino acids
genetic code expansion
crystal structure
tRNA
cell-free protein synthesis
Online Access:https://www.mdpi.com/1422-0067/24/7/6256

Internet

https://www.mdpi.com/1422-0067/24/7/6256

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