The bacterial hitchhiker’s guide to COI: Universal primer-based COI capture probes fail to exclude bacterial DNA, but 16S capture leaves metazoa behind

Environmental DNA (eDNA) metabarcoding from water samples has, in recent years, shown great promise for biodiversity monitoring. However, universal primers targeting the cytochrome oxidase I (COI) marker gene popular in metazoan studies have displayed high levels of nontarget amplification. To date,...

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Bibliographic Details
Main Authors: Sanni Hintikka, Jeanette E.L. Carlsson, Jens Carlsson
Format: Article
Language:English
Published: Pensoft Publishers 2022-05-01
Series:Metabarcoding and Metagenomics
Online Access:https://mbmg.pensoft.net/article/80416/download/pdf/