Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation
Boletus aereus, an edible mushroom, has gained popularity as a medicinal and functional food. This study aimed to investigate the digestive characteristics of B. aereus polysaccharide (BAP) and its effects on gut microbiota. In vitro digestion results indicated partial degradation of BAP. Furthermor...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523004959 |
| _version_ | 1797244939136925696 |
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| author | Shixiang Wei Luanfeng Wang Xiaodie Chen Yue Wang Lingling Tong Qianyun Han Bo Ren Dongsheng Guo |
| author_facet | Shixiang Wei Luanfeng Wang Xiaodie Chen Yue Wang Lingling Tong Qianyun Han Bo Ren Dongsheng Guo |
| author_sort | Shixiang Wei |
| collection | DOAJ |
| description | Boletus aereus, an edible mushroom, has gained popularity as a medicinal and functional food. This study aimed to investigate the digestive characteristics of B. aereus polysaccharide (BAP) and its effects on gut microbiota. In vitro digestion results indicated partial degradation of BAP. Furthermore, the digested BAP displayed significantly enhanced antioxidant ability. The 16S rRNA sequencing data revealed that BAP positively influenced the abundance of Phascolarctobacterium, Prevotella, and Bifidobacterium in the gut microbiota. Additionally, BAP promoted the production of short-chain fatty acids (SCFAs). Metabolites of BAP utilized by the gut microbiota effectively reduced the concentration of TNF-α, IL-1β, and NO in an LPS-stimulated RAW 264.7 cell inflammation model. Mantel tests demonstrated a strong correlation among fermentation indicators, gut microbiome composition, SCFAs, and inflammatory cytokines. Overall, this research revealed the underlying digestive and fermentation mechanisms of BAP and provided new insights into the usage of edible mushroom polysaccharides in functional food. |
| first_indexed | 2024-03-08T21:50:19Z |
| format | Article |
| id | doaj.art-c0e5abe86ae344739dc2ccc08e7b5c42 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-24T19:18:58Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-c0e5abe86ae344739dc2ccc08e7b5c422024-03-26T04:27:02ZengElsevierFood Chemistry: X2590-15752024-03-0121101052Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentationShixiang Wei0Luanfeng Wang1Xiaodie Chen2Yue Wang3Lingling Tong4Qianyun Han5Bo Ren6Dongsheng Guo7School of Food Science and Pharmaceutical Engineering, Nanjing Normal University Nanjing 210023, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210023, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University Nanjing 210023, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University Nanjing 210023, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University Nanjing 210023, ChinaBIOSYST-MeBioS, Faculty of Bioscience Engineering, KU Leuven, Leuven 3000, Belgium; College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Beijing 100083, ChinaSchool of Food Science and Pharmaceutical Engineering, Nanjing Normal University Nanjing 210023, China; Corresponding authors.School of Food Science and Pharmaceutical Engineering, Nanjing Normal University Nanjing 210023, China; Corresponding authors.Boletus aereus, an edible mushroom, has gained popularity as a medicinal and functional food. This study aimed to investigate the digestive characteristics of B. aereus polysaccharide (BAP) and its effects on gut microbiota. In vitro digestion results indicated partial degradation of BAP. Furthermore, the digested BAP displayed significantly enhanced antioxidant ability. The 16S rRNA sequencing data revealed that BAP positively influenced the abundance of Phascolarctobacterium, Prevotella, and Bifidobacterium in the gut microbiota. Additionally, BAP promoted the production of short-chain fatty acids (SCFAs). Metabolites of BAP utilized by the gut microbiota effectively reduced the concentration of TNF-α, IL-1β, and NO in an LPS-stimulated RAW 264.7 cell inflammation model. Mantel tests demonstrated a strong correlation among fermentation indicators, gut microbiome composition, SCFAs, and inflammatory cytokines. Overall, this research revealed the underlying digestive and fermentation mechanisms of BAP and provided new insights into the usage of edible mushroom polysaccharides in functional food.http://www.sciencedirect.com/science/article/pii/S2590157523004959Boletus aereusPolysaccharidesIn vitro digestionGut microbiotaAnti-inflammatory activity |
| spellingShingle | Shixiang Wei Luanfeng Wang Xiaodie Chen Yue Wang Lingling Tong Qianyun Han Bo Ren Dongsheng Guo Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation Food Chemistry: X Boletus aereus Polysaccharides In vitro digestion Gut microbiota Anti-inflammatory activity |
| title | Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation |
| title_full | Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation |
| title_fullStr | Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation |
| title_full_unstemmed | Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation |
| title_short | Anti-inflammatory activity of Boletus aereus polysaccharides: Involvement of digestion and gut microbiota fermentation |
| title_sort | anti inflammatory activity of boletus aereus polysaccharides involvement of digestion and gut microbiota fermentation |
| topic | Boletus aereus Polysaccharides In vitro digestion Gut microbiota Anti-inflammatory activity |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523004959 |
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