Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients
Objective: To analyze global DNA methylation along with DNA methyltransferases (DNMTs) expression at transcript level in patients with impaired spermatogenesis to dissect its role in pathophysiology of human male infertility. Method: The content of Global methylated cytosine (mC) was determined usin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-12-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050015000214 |
| _version_ | 1818197486238433280 |
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| author | Deepika Jaiswal Sameer Trivedi Neeraj K. Agrawal Kiran Singh |
| author_facet | Deepika Jaiswal Sameer Trivedi Neeraj K. Agrawal Kiran Singh |
| author_sort | Deepika Jaiswal |
| collection | DOAJ |
| description | Objective: To analyze global DNA methylation along with DNA methyltransferases (DNMTs) expression at transcript level in patients with impaired spermatogenesis to dissect its role in pathophysiology of human male infertility.
Method: The content of Global methylated cytosine (mC) was determined using ELISA system (Imprint Methylated DNA Quantification Kit, Sigma–Aldrich) in 31 testicular biopsies showing impaired spermatogenesis and 8 with normal spermatogenesis. Real-time reverse transcription-polymerase chain reaction was done to analyze DNMTs (DNMT1, DNMT3A, DNMT3B and DNMT3L) mRNA levels in biopsies with different testicular phenotypes.
Results: There was significant increase in levels of global methylation in different impaired testicular phenotypes as compared to normal. Expression analysis revealed significantly increased expression of DNMT1 and its positive correlation with global DNA methylation.
Conclusion: In conclusion, increased levels of global methylation in impaired cases might be the one of the contributing factors for aberrant gene expression in infertile patients. |
| first_indexed | 2024-12-12T01:50:44Z |
| format | Article |
| id | doaj.art-c0ff994e01d740598adececbb825aa51 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 |
| language | English |
| last_indexed | 2024-12-12T01:50:44Z |
| publishDate | 2015-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-c0ff994e01d740598adececbb825aa512022-12-22T00:42:29ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002015-12-014426226510.1016/j.apjr.2015.07.003Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patientsDeepika Jaiswal0Sameer Trivedi1Neeraj K. Agrawal2Kiran Singh3Department of Molecular and Human Genetics, Banaras Hindu University, Varanasi, IndiaDepartment of Urology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Endocrinology and Metabolism, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Molecular and Human Genetics, Banaras Hindu University, Varanasi, IndiaObjective: To analyze global DNA methylation along with DNA methyltransferases (DNMTs) expression at transcript level in patients with impaired spermatogenesis to dissect its role in pathophysiology of human male infertility. Method: The content of Global methylated cytosine (mC) was determined using ELISA system (Imprint Methylated DNA Quantification Kit, Sigma–Aldrich) in 31 testicular biopsies showing impaired spermatogenesis and 8 with normal spermatogenesis. Real-time reverse transcription-polymerase chain reaction was done to analyze DNMTs (DNMT1, DNMT3A, DNMT3B and DNMT3L) mRNA levels in biopsies with different testicular phenotypes. Results: There was significant increase in levels of global methylation in different impaired testicular phenotypes as compared to normal. Expression analysis revealed significantly increased expression of DNMT1 and its positive correlation with global DNA methylation. Conclusion: In conclusion, increased levels of global methylation in impaired cases might be the one of the contributing factors for aberrant gene expression in infertile patients.http://www.sciencedirect.com/science/article/pii/S2305050015000214MethylationMale infertilityMethyltransferasesExpressionSpermatogenesisTestis |
| spellingShingle | Deepika Jaiswal Sameer Trivedi Neeraj K. Agrawal Kiran Singh Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients Asian Pacific Journal of Reproduction Methylation Male infertility Methyltransferases Expression Spermatogenesis Testis |
| title | Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients |
| title_full | Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients |
| title_fullStr | Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients |
| title_full_unstemmed | Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients |
| title_short | Association of the patterns of global DNA methylation and expression analysis of DNA methyltransferases in impaired spermatogenic patients |
| title_sort | association of the patterns of global dna methylation and expression analysis of dna methyltransferases in impaired spermatogenic patients |
| topic | Methylation Male infertility Methyltransferases Expression Spermatogenesis Testis |
| url | http://www.sciencedirect.com/science/article/pii/S2305050015000214 |
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