Detection of Klebsiella pneumonia DNA and ESBL positive strains by PCR-based CRISPR-LbCas12a system

Detection of Klebsiella pneumonia DNA and ESBL positive strains by PCR-based CRISPR-LbCas12a system

IntroductionKlebsiella pneumonia (K. pneumonia) is a Gram-negative bacterium that opportunistically causes nosocomial infections in the lung, bloodstream, and urinary tract. Extended-spectrum β-Lactamases (ESBLs)-expressed K. pneumonia strains are widely reported to cause antibiotic resistance and t...

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Bibliographic Details
Main Authors: Shang Wang, Shan Wang, Ying Tang, Guoyu Peng, Tongyu Hao, Xincheng Wu, Jiehong Wei, Xinying Qiu, Dewang Zhou, Shimao Zhu, Yuqing Li, Song Wu
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-02-01
Series:Frontiers in Microbiology
Subjects:
CRISPR-Cas
nucleic acid detection
Klebsiella pneumonia (K. pneumonia)
Extended-spectrum β-lactamases (ESBL)
SHV
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128261/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128261/full

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