Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates
Background: Agrowastes like Theobroma cacao (Cocoa) pod husk can be used to prepare bioactive peptides with various bio-functionalities. Objectives: This study aimed to investigate antioxidant and angiotensin converting enzyme I (ACE) inhibitory peptides contained in Theobroma cacao (cocoa) pod h...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hamadan University of Medical Sciences
2021-06-01
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| Series: | Avicenna Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | http://ajmb.umsha.ac.ir/PDF/ajmb-9-26.pdf |
| _version_ | 1819195397414846464 |
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| author | Rotimi Arise Samuel Tobi Farohunbi Halimat Olanike Ayilara |
| author_facet | Rotimi Arise Samuel Tobi Farohunbi Halimat Olanike Ayilara |
| author_sort | Rotimi Arise |
| collection | DOAJ |
| description | Background: Agrowastes like Theobroma cacao (Cocoa) pod husk can be used to prepare bioactive peptides with various bio-functionalities.
Objectives: This study aimed to investigate antioxidant and angiotensin converting enzyme I (ACE) inhibitory peptides contained in Theobroma cacao (cocoa) pod husks – an agro-waste.
Methods: Protein isolated from cocoa pod husk was enzymatically digested with alcalase, pepsin, and trypsin. ACE inhibition, kinetics of ACE inhibition, and antioxidant properties of the cocoa pod husks hydrolysates were evaluated in vitro.
Results: Trypsin and alcalase hydrolysates displayed higher peptide yields (63.1% and 61.2%) than pepsin hydrolysate (61.2%). However, no significant difference (P>0.05) was observed in the degree of hydrolysis (DH) of the three proteases on cocoa pod husk protein. Methionine, lysine, and cysteine were the amino acid residues presented in cocoa pod husk hydrolysates. A concentration-dependent ACE inhibition by cocoa pod husk hydrolysates was observed. The highest ACE inhibitions of 84.4%, 81.5%, and 73.5% were obtained at 2.0 mg/mL of pepsin, trypsin, and alcalase hydrolysates, respectively, with the minimum IC50 value of 0.36 mg/mL obtained for trypsin hydrolysate. An uncompetitive and mixed-type inhibition was obtained from double reciprocal plots of alcalase and pepsin as well as trypsin cocoa pod husk protein hydrolysates. The Ki values of ACE inhibition for pepsin, trypsin, and alcalase hydrolysates were 3.05, 2.19, and 3.57 mg/mL, respectively. A concentration-dependent increase in the scavenging of 2,2-diphenyl-1-picrylhydrazyl and superoxide radicals as well as ferric reducing antioxidant power were recorded for the cocoa pod husk hydrolysates.
Conclusion: Trypsin and alcalase cocoa pod husk protein hydrolysates could be an effective source of a natural ACE inhibitor and antioxidant. |
| first_indexed | 2024-12-23T02:12:07Z |
| format | Article |
| id | doaj.art-c129a73da0c54cc99ee3b7318628a72f |
| institution | Directory Open Access Journal |
| issn | 2345-4113 |
| language | English |
| last_indexed | 2024-12-23T02:12:07Z |
| publishDate | 2021-06-01 |
| publisher | Hamadan University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Medical Biochemistry |
| spelling | doaj.art-c129a73da0c54cc99ee3b7318628a72f2022-12-21T18:03:46ZengHamadan University of Medical SciencesAvicenna Journal of Medical Biochemistry2345-41132021-06-0191263610.34172/ajmb.2021.05Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein HydrolysatesRotimi Arise0Samuel Tobi Farohunbi1https://orcid.org/0000-0001-8400-169XHalimat Olanike Ayilara2Department of Biochemistry, University of Ilorin, Ilorin, NigeriaDepartment of Biological Sciences, Thomas Adewumi University, Oko, NigeriaDepartment of Biochemistry, University of Ilorin, Ilorin, NigeriaBackground: Agrowastes like Theobroma cacao (Cocoa) pod husk can be used to prepare bioactive peptides with various bio-functionalities. Objectives: This study aimed to investigate antioxidant and angiotensin converting enzyme I (ACE) inhibitory peptides contained in Theobroma cacao (cocoa) pod husks – an agro-waste. Methods: Protein isolated from cocoa pod husk was enzymatically digested with alcalase, pepsin, and trypsin. ACE inhibition, kinetics of ACE inhibition, and antioxidant properties of the cocoa pod husks hydrolysates were evaluated in vitro. Results: Trypsin and alcalase hydrolysates displayed higher peptide yields (63.1% and 61.2%) than pepsin hydrolysate (61.2%). However, no significant difference (P>0.05) was observed in the degree of hydrolysis (DH) of the three proteases on cocoa pod husk protein. Methionine, lysine, and cysteine were the amino acid residues presented in cocoa pod husk hydrolysates. A concentration-dependent ACE inhibition by cocoa pod husk hydrolysates was observed. The highest ACE inhibitions of 84.4%, 81.5%, and 73.5% were obtained at 2.0 mg/mL of pepsin, trypsin, and alcalase hydrolysates, respectively, with the minimum IC50 value of 0.36 mg/mL obtained for trypsin hydrolysate. An uncompetitive and mixed-type inhibition was obtained from double reciprocal plots of alcalase and pepsin as well as trypsin cocoa pod husk protein hydrolysates. The Ki values of ACE inhibition for pepsin, trypsin, and alcalase hydrolysates were 3.05, 2.19, and 3.57 mg/mL, respectively. A concentration-dependent increase in the scavenging of 2,2-diphenyl-1-picrylhydrazyl and superoxide radicals as well as ferric reducing antioxidant power were recorded for the cocoa pod husk hydrolysates. Conclusion: Trypsin and alcalase cocoa pod husk protein hydrolysates could be an effective source of a natural ACE inhibitor and antioxidant.http://ajmb.umsha.ac.ir/PDF/ajmb-9-26.pdftheobroma cacao pod huskprotein hydrolysatesace inhibitionantioxidants |
| spellingShingle | Rotimi Arise Samuel Tobi Farohunbi Halimat Olanike Ayilara Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates Avicenna Journal of Medical Biochemistry theobroma cacao pod husk protein hydrolysates ace inhibition antioxidants |
| title | Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates |
| title_full | Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates |
| title_fullStr | Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates |
| title_full_unstemmed | Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates |
| title_short | Blood Pressure Regulating and Antioxidant Potentials of Theobroma Cacao Pod Husk Protein Hydrolysates |
| title_sort | blood pressure regulating and antioxidant potentials of theobroma cacao pod husk protein hydrolysates |
| topic | theobroma cacao pod husk protein hydrolysates ace inhibition antioxidants |
| url | http://ajmb.umsha.ac.ir/PDF/ajmb-9-26.pdf |
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