A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I

We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) comple...

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Bibliographic Details
Main Authors: Gang Liu, Lihua Wang, Jingwen Chen, Shuzhen Ren, Dun Pan, Quanwen Liu, Hui Xu, Shuli Gao, Min Ding
Format: Article
Language:English
Published: MDPI AG 2011-10-01
Series:Sensors
Subjects:
cysteine
competition assay
mercury-specific DNA
Hg2+
Sybr Green I
Online Access:http://www.mdpi.com/1424-8220/11/11/10187/

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http://www.mdpi.com/1424-8220/11/11/10187/

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